This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MGP M155
catalog :
26527
citations: 2
Reference
Monnot G, Martinez Usatorre A, Lanitis E, Lopes S, Cheng W, Ho P, et al. miR-155 Overexpression in OT-1 CD8+ T Cells Improves Anti-Tumor Activity against Low-Affinity Tumor Antigen. Mol Ther Oncolytics. 2020;16:111-123 pubmed publisher
O Connell R, Chaudhuri A, Rao D, Baltimore D. Inositol phosphatase SHIP1 is a primary target of miR-155. Proc Natl Acad Sci U S A. 2009;106:7113-8 pubmed publisher
product information
Catalog Number :
26527
Product Name :
MGP M155
article :
doi10.1073/pnas.0902636106
id3685
pubmed_id19359473
bacterial resistance :
Ampicillin
cloning :
backbonepMSCV puro
backbone_mutation
backbone_origin
backbone_size6300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Retroviral
growth notes :
pMGP is a modified MSCV vector whereby GFP was placed downstream from the 5 LTR, and the miR-155 expression cassette was cloned downstream from the GFP stop codon.
origin :
37
pi :
alt_names
BIC (miRNA containing region of Exon2)
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NotI
promoter
sequencing_primer_3MSCV-rev
sequencing_primer_5pLXSN-5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMirn155, mir-155, mmu-mir-155
geneMir155
id387173
genbank_ids
mutation
namemiR-155
shRNA_sequence
size
species
10090
Mus musculus
tags
locationN terminal on backbone
tagGFP
resistance markers :
20
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA