This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-PCNA-IRES-puro2b
catalog :
26461
citations: 5
Reference
Rhodes J, Haarhuis J, Grimm J, Rowland B, Lavis L, Nasmyth K. Cohesin Can Remain Associated with Chromosomes during DNA Replication. Cell Rep. 2017;20:2749-2755 pubmed publisher
Waraky A, Lin Y, Warsito D, Haglund F, Aleem E, Larsson O. Nuclear insulin-like growth factor 1 receptor phosphorylates proliferating cell nuclear antigen and rescues stalled replication forks after DNA damage. J Biol Chem. 2017;292:18227-18239 pubmed publisher
Gudipaty S, Lindblom J, Loftus P, Redd M, Edes K, Davey C, et al. Mechanical stretch triggers rapid epithelial cell division through Piezo1. Nature. 2017;543:118-121 pubmed publisher
Greil C, Krohs J, Schnerch D, Follo M, Felthaus J, Engelhardt M, et al. The role of APC/C(Cdh1) in replication stress and origin of genomic instability. Oncogene. 2016;35:3062-70 pubmed publisher
Held M, Schmitz M, Fischer B, Walter T, Neumann B, Olma M, et al. CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging. Nat Methods. 2010;7:747-54 pubmed publisher
product information
Catalog Number :
26461
Product Name :
pEGFP-PCNA-IRES-puro2b
article :
doi10.1038/nmeth.1486
id3679
pubmed_id20693996
bacterial resistance :
Ampicillin
cloning :
backbonepIRES-puro2b
backbone_mutation
backbone_origin
backbone_size6037
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
growth notes :
Note that there are a few minor discrepancies between Addgene's quality control sequence and the author's sequence, but these should not affect function.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Xba1
cloning_site_5Nde1
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATLD2
genePCNA
id5111
genbank_ids
NP_002583.1
mutation
namePCNA
shRNA_sequence
size758
species
9606
Homo sapiens
tags
locationN terminal on insert
tagpEGFP
resistance markers :
162
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA