This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pF146 pSOD1WTAcGFP1
catalog :
26407
citations: 9
Reference
Norambuena A, Sun X, Wallrabe H, Cao R, Sun N, Pardo E, et al. SOD1 mediates lysosome-to-mitochondria communication and its dysregulation by amyloid-β oligomers. Neurobiol Dis. 2022;169:105737 pubmed publisher
Li X, Chen C, Zhan X, Li B, Zhang Z, Li S, et al. R13 preserves motor performance in SOD1G93A mice by improving mitochondrial function. Theranostics. 2021;11:7294-7307 pubmed publisher
Akieda Y, Ogamino S, Furuie H, Ishitani S, Akiyoshi R, Nogami J, et al. Cell competition corrects noisy Wnt morphogen gradients to achieve robust patterning in the zebrafish embryo. Nat Commun. 2019;10:4710 pubmed publisher
Tsang C, Chen M, Cheng X, Qi Y, Chen Y, Das I, et al. SOD1 Phosphorylation by mTORC1 Couples Nutrient Sensing and Redox Regulation. Mol Cell. 2018;70:502-515.e8 pubmed publisher
Malty R, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, et al. A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB Signaling. Cell Syst. 2017;5:564-577.e12 pubmed publisher
Cruz Garcia D, Brouwers N, Duran J, Mora G, Curwin A, Malhotra V. A diacidic motif determines unconventional secretion of wild-type and ALS-linked mutant SOD1. J Cell Biol. 2017;: pubmed publisher
Matějů D, Franzmann T, Patel A, Kopach A, Boczek E, Maharana S, et al. An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function. EMBO J. 2017;36:1669-1687 pubmed publisher
Liu Y, Miao K, Dunham N, Liu H, Fares M, Boal A, et al. The Cation-π Interaction Enables a Halo-Tag Fluorogenic Probe for Fast No-Wash Live Cell Imaging and Gel-Free Protein Quantification. Biochemistry. 2017;56:1585-1595 pubmed publisher
Stevens J, Chia R, Hendriks W, Bros Facer V, van Minnen J, Martin J, et al. Modification of superoxide dismutase 1 (SOD1) properties by a GFP tag--implications for research into amyotrophic lateral sclerosis (ALS). PLoS ONE. 2010;5:e9541 pubmed publisher
product information
Catalog Number :
26407
Product Name :
pF146 pSOD1WTAcGFP1
article :
doi10.1371/journal.pone.0009541
id3658
pubmed_id20221404
bacterial resistance :
Kanamycin
cloning :
backbonepAcGFP1-N1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The complete cDNA was subcloned into the vector pET28 and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5 and 3 end respectively. This EcoRI-Sal1 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pAcGFP1N1. Thus SOD1 is 5 of GFP and SOD1 lies upstream of GFP in the SOD1-GFP fusion protein.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer
geneSOD1
id6647
genbank_ids
mutation
nameSOD1
shRNA_sequence
size470
species
9606
Homo sapiens
tags
locationC terminal on insert
tagGFP
plasmid copy :
human cDNA insert from cDNA clone cSOD1 from Professor Yoram Groner as described in Lieman-Hurwitzet al., 1982 and Sherman et al 1983.
resistance markers :
804
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA