This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pF143 pAcGFP1 SOD1G37R
catalog :
26404
citations: 1
product information
Catalog Number :
26404
Product Name :
pF143 pAcGFP1 SOD1G37R
article :
| doi | 10.1371/journal.pone.0009541 |
| id | 3658 |
| pubmed_id | 20221404 |
bacterial resistance :
Kanamycin
cloning :
| backbone | pAcGFP1-C1 | |
| backbone_mutation | ||
| backbone_origin | Clontech | |
| backbone_size | 4700 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
The complete cDNA was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 37 of the cDNA from GGA to AGA (*see Addgene's note below), resulting in the G37R mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5 and 3 end respectively. This EcoRI-Sal1 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pACGFPC1. Thus GFP is 5 of the SOD1 insert and upstream in the GFP-SOD1 fusion protein. *Please note that the Addgene verified sequence found that codon 37 was mutated to CGT which still encodes Arginine.
origin :
37
pi :
|
plasmid copy :
human cDNA insert from cDNA clone cSOD1 from Professor Yoram Groner as described in Lieman-Hurwitzet al., 1982 and Sherman et al 1983.
resistance markers :
| 804 |
tags :
Unknown
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments