This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pF141 pAcGFP1 SOD1WT
catalog :
26402
citations: 7
Reference
Dubey A, Mishra R, Jagtap Y, Kinger S, Kumar P, Dhiman R, et al. Itraconazole Confers Cytoprotection Against Neurodegenerative Disease-Associated Abnormal Protein Aggregation. Mol Neurobiol. 2023;60:2397-2412 pubmed publisher
Naqvi S, Khanadeev V, Khlebtsov B, Khlebtsov N, Deore M, Packirisamy G. Albumin-Based Nanocarriers for the Simultaneous Delivery of Antioxidant Gene and Phytochemical to Combat Oxidative Stress. Front Cell Dev Biol. 2022;10:846175 pubmed publisher
Liu X, Zhang J, Li J, Song C, Shi Y. Pharmacological inhibition of ALCAT1 mitigates amyotrophic lateral sclerosis by attenuating SOD1 protein aggregation. Mol Metab. 2022;63:101536 pubmed publisher
Woo T, Yoon M, Kang S, Park S, Cho J, Hwang Y, et al. Novel chemical inhibitor against SOD1 misfolding and aggregation protects neuron-loss and ameliorates disease symptoms in ALS mouse model. Commun Biol. 2021;4:1397 pubmed publisher
Joshi A, Minhas P, Liddelow S, Haileselassie B, Andreasson K, Dorn G, et al. Fragmented mitochondria released from microglia trigger A1 astrocytic response and propagate inflammatory neurodegeneration. Nat Neurosci. 2019;22:1635-1648 pubmed publisher
Upadhyay A, Amanullah A, Mishra R, Kumar A, Mishra A. Lanosterol Suppresses the Aggregation and Cytotoxicity of Misfolded Proteins Linked with Neurodegenerative Diseases. Mol Neurobiol. 2018;55:1169-1182 pubmed publisher
Stevens J, Chia R, Hendriks W, Bros Facer V, van Minnen J, Martin J, et al. Modification of superoxide dismutase 1 (SOD1) properties by a GFP tag--implications for research into amyotrophic lateral sclerosis (ALS). PLoS ONE. 2010;5:e9541 pubmed publisher
product information
Catalog Number :
26402
Product Name :
pF141 pAcGFP1 SOD1WT
article :
doi10.1371/journal.pone.0009541
id3658
pubmed_id20221404
bacterial resistance :
Kanamycin
cloning :
backbonepAcGFP1-C1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The complete cDNA was subcloned into the vector pET28 and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5 and 3 end respectively. This EcoRI-SalI 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pACGFPC1. Thus GFP is 5 of the SOD1 insert and upstream in the GFP-SOD1 fusion protein.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoter
sequencing_primer_3SV40pA-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer
geneSOD1
id6647
genbank_ids
mutation
nameSOD1
shRNA_sequence
size470
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGFP
plasmid copy :
human cDNA insert from cDNA clone cSOD1 from Professor Yoram Groner as described in Lieman-Hurwitzet al., 1982 and Sherman et al 1983.
resistance markers :
804
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA