pF155 pcDNA3.1(+)SOD1G93A | Addgene 26401 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pF155 pcDNA3.1(+)SOD1G93A

catalog :

26401

citations: 7

Reference
Chen X, He X, Yang Y, Wang Y. Amyotrophic Lateral Sclerosis-Associated Mutants of SOD1 Modulate miRNA Biogenesis through Aberrant Interactions with Exportin 5. ACS Chem Biol. 2022;17:3450-3457 pubmed publisher
Woo T, Yoon M, Kang S, Park S, Cho J, Hwang Y, et al. Novel chemical inhibitor against SOD1 misfolding and aggregation protects neuron-loss and ameliorates disease symptoms in ALS mouse model. Commun Biol. 2021;4:1397 pubmed publisher
Guida N, Sanguigno L, Mascolo L, Calabrese L, Serani A, Molinaro P, et al. The Transcriptional Complex Sp1/KMT2A by Up-Regulating Restrictive Element 1 Silencing Transcription Factor Accelerates Methylmercury-Induced Cell Death in Motor Neuron-Like NSC34 Cells Overexpressing SOD1-G93A. Front Neurosci. 2021;15:771580 pubmed publisher
Laudati G, Mascolo L, Guida N, Sirabella R, Pizzorusso V, Bruzzaniti S, et al. Resveratrol treatment reduces the vulnerability of SH-SY5Y cells and cortical neurons overexpressing SOD1-G93A to Thimerosal toxicity through SIRT1/DREAM/PDYN pathway. Neurotoxicology. 2019;71:6-15 pubmed publisher
Halon Golabek M, Borkowska A, Kaczor J, Ziolkowski W, Flis D, Knap N, et al. hmSOD1 gene mutation-induced disturbance in iron metabolism is mediated by impairment of Akt signalling pathway. J Cachexia Sarcopenia Muscle. 2018;9:557-569 pubmed publisher
Gaj T, Ojala D, Ekman F, Byrne L, Limsirichai P, Schaffer D. In vivo genome editing improves motor function and extends survival in a mouse model of ALS. Sci Adv. 2017;3:eaar3952 pubmed publisher
Stevens J, Chia R, Hendriks W, Bros Facer V, van Minnen J, Martin J, et al. Modification of superoxide dismutase 1 (SOD1) properties by a GFP tag--implications for research into amyotrophic lateral sclerosis (ALS). PLoS ONE. 2010;5:e9541 pubmed publisher

product information

Catalog Number :

26401

Product Name :

pF155 pcDNA3.1(+)SOD1G93A

article :

doi	10.1371/journal.pone.0009541
id	3658
pubmed_id	20221404

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1 (+)

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The complete cDNA insert was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 93 of the cDNA from GGT to GCT, resulting in the G93A mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and XhoI sites on the 5 and 3 end respectively. This EcoRI-Xho1 0.47kb fragment was cloned into the respective EcoRI and XhoI sites of pcDNA3.1(+).

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer
gene	SOD1
id	6647

genbank_ids

mutation

G93A

name

SOD1

shRNA_sequence

size

470

species

9606

Homo sapiens