This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pF154 pcDNA3.1(+)SOD1G85R
catalog :
26400
citations: 3
Reference
Chen X, He X, Yang Y, Wang Y. Amyotrophic Lateral Sclerosis-Associated Mutants of SOD1 Modulate miRNA Biogenesis through Aberrant Interactions with Exportin 5. ACS Chem Biol. 2022;17:3450-3457 pubmed publisher
Woo T, Yoon M, Kang S, Park S, Cho J, Hwang Y, et al. Novel chemical inhibitor against SOD1 misfolding and aggregation protects neuron-loss and ameliorates disease symptoms in ALS mouse model. Commun Biol. 2021;4:1397 pubmed publisher
Stevens J, Chia R, Hendriks W, Bros Facer V, van Minnen J, Martin J, et al. Modification of superoxide dismutase 1 (SOD1) properties by a GFP tag--implications for research into amyotrophic lateral sclerosis (ALS). PLoS ONE. 2010;5:e9541 pubmed publisher
product information
Catalog Number :
26400
Product Name :
pF154 pcDNA3.1(+)SOD1G85R
article :
doi10.1371/journal.pone.0009541
id3658
pubmed_id20221404
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1 (+)
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The complete cDNA insert was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 85 of the cDNA from GGC to CGC, resulting in the G85R mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and XhoI sites on the 5 and 3 end respectively. This EcoRI-Xho1 0.47kb fragment was cloned into the respective EcoRI and XhoI sites of pcDNA3.1(+).
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer
geneSOD1
id6647
genbank_ids
mutationG85R
nameSOD1
shRNA_sequence
size470
species
9606
Homo sapiens
tags
plasmid copy :
human cDNA insert from cDNA clone cSOD1 from Professor Yoram Groner as described in Lieman-Hurwitzet al., 1982 and Sherman et al 1983.
resistance markers :
804
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA