pF152 pcDNA3.1(+)SOD1A4V | Addgene 26398 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pF152 pcDNA3.1(+)SOD1A4V

catalog :

26398

citations: 2

Reference
Woo T, Yoon M, Kang S, Park S, Cho J, Hwang Y, et al. Novel chemical inhibitor against SOD1 misfolding and aggregation protects neuron-loss and ameliorates disease symptoms in ALS mouse model. Commun Biol. 2021;4:1397 pubmed publisher
Stevens J, Chia R, Hendriks W, Bros Facer V, van Minnen J, Martin J, et al. Modification of superoxide dismutase 1 (SOD1) properties by a GFP tag--implications for research into amyotrophic lateral sclerosis (ALS). PLoS ONE. 2010;5:e9541 pubmed publisher

product information

Catalog Number :

26398

Product Name :

pF152 pcDNA3.1(+)SOD1A4V

article :

doi	10.1371/journal.pone.0009541
id	3658
pubmed_id	20221404

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1 (+)

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The complete cDNA insert was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 4 of the cDNA from GCC to GTC, resulting in the A4V mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and XhoI sites on the 5 and 3 end respectively. This EcoRI-Xho1 0.47kb fragment was cloned into the respective EcoRI and XhoI sites of pcDNA3.1(+).

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer
gene	SOD1
id	6647

genbank_ids

mutation

A4V

name

SOD1

shRNA_sequence

size

470

species

9606

Homo sapiens