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product summary
company name :
Addgene
product type :
cDNA
product name :
pF151 pcDNA3.1(+)SOD1WT
catalog :
26397
citations: 11
Reference |
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Guida N, Sanguigno L, Mascolo L, Calabrese L, Serani A, Molinaro P, et al. The Transcriptional Complex Sp1/KMT2A by Up-Regulating Restrictive Element 1 Silencing Transcription Factor Accelerates Methylmercury-Induced Cell Death in Motor Neuron-Like NSC34 Cells Overexpressing SOD1-G93A. Front Neurosci. 2021;15:771580 pubmed publisher
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product information
Catalog Number :
26397
Product Name :
pF151 pcDNA3.1(+)SOD1WT
article :
doi | 10.1371/journal.pone.0009541 |
id | 3658 |
pubmed_id | 20221404 |
bacterial resistance :
Ampicillin
cloning :
backbone | pcDNA3.1 (+) | |
backbone_mutation | ||
backbone_origin | Invitrogen | |
backbone_size | 5400 | |
promoter | ||
sequencing_primer_3 | ||
sequencing_primer_5 | ||
vector_types |
|
growth notes :
. The complete cDNA insert was subcloned into the vector pET28 and then a PCR amplified the complete cDNA with EcoRI and XhoI sites on the 5 and 3 end respectively. This EcoRI-Xho1 0.47kb fragment was cloned into the respective EcoRI and XhoI sites of pcDNA3.1(+).
origin :
37
pi :
|
plasmid copy :
human cDNA insert from cDNA clone cSOD1 from Professor Yoram Groner as described in Lieman-Hurwitzet al., 1982 and Sherman et al 1983.
resistance markers :
804 |
tags :
High Copy
terms :
Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
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