This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pF151 pcDNA3.1(+)SOD1WT
catalog :
26397
citations: 11
Reference
Chen H, Chen T. Probing Oxidant Effects on Superoxide Dismutase 1 Oligomeric States in Live Cells Using Single-Molecule Fluorescence Anisotropy. Chem Biomed Eng. 2023;1:49-57 pubmed publisher
Baek Y, Woo T, Ahn J, Lee D, Kwon Y, Park B, et al. Structural analysis of the overoxidized Cu/Zn-superoxide dismutase in ROS-induced ALS filament formation. Commun Biol. 2022;5:1085 pubmed publisher
Carmona Rodríguez L, Martínez Rey D, Martín González P, Franch M, Sorokin L, Mira E, et al. Superoxide Dismutase-3 Downregulates Laminin α5 Expression in Tumor Endothelial Cells via the Inhibition of Nuclear Factor Kappa B Signaling. Cancers (Basel). 2022;14: pubmed publisher
Woo T, Yoon M, Kang S, Park S, Cho J, Hwang Y, et al. Novel chemical inhibitor against SOD1 misfolding and aggregation protects neuron-loss and ameliorates disease symptoms in ALS mouse model. Commun Biol. 2021;4:1397 pubmed publisher
Guida N, Sanguigno L, Mascolo L, Calabrese L, Serani A, Molinaro P, et al. The Transcriptional Complex Sp1/KMT2A by Up-Regulating Restrictive Element 1 Silencing Transcription Factor Accelerates Methylmercury-Induced Cell Death in Motor Neuron-Like NSC34 Cells Overexpressing SOD1-G93A. Front Neurosci. 2021;15:771580 pubmed publisher
Strong M, Hart M, Tang T, Ojo B, Wu L, Nacke M, et al. Role of zinc transporter ZIP12 in susceptibility-weighted brain magnetic resonance imaging (MRI) phenotypes and mitochondrial function. FASEB J. 2020;34:10702-12725 pubmed publisher
Du T, Song Y, Ray A, Chauhan D, Anderson K. Proteomic analysis identifies mechanism(s) of overcoming bortezomib resistance via targeting ubiquitin receptor Rpn13. Leukemia. 2020;: pubmed publisher
Sharif T, Dai C, Martell E, Ghassemi Rad M, Hanes M, Murphy P, et al. TAp73 Modifies Metabolism and Positively Regulates Growth of Cancer Stem-Like Cells in a Redox-Sensitive Manner. Clin Cancer Res. 2019;25:2001-2017 pubmed publisher
Laudati G, Mascolo L, Guida N, Sirabella R, Pizzorusso V, Bruzzaniti S, et al. Resveratrol treatment reduces the vulnerability of SH-SY5Y cells and cortical neurons overexpressing SOD1-G93A to Thimerosal toxicity through SIRT1/DREAM/PDYN pathway. Neurotoxicology. 2019;71:6-15 pubmed publisher
Halon Golabek M, Borkowska A, Kaczor J, Ziolkowski W, Flis D, Knap N, et al. hmSOD1 gene mutation-induced disturbance in iron metabolism is mediated by impairment of Akt signalling pathway. J Cachexia Sarcopenia Muscle. 2018;9:557-569 pubmed publisher
Stevens J, Chia R, Hendriks W, Bros Facer V, van Minnen J, Martin J, et al. Modification of superoxide dismutase 1 (SOD1) properties by a GFP tag--implications for research into amyotrophic lateral sclerosis (ALS). PLoS ONE. 2010;5:e9541 pubmed publisher
product information
Catalog Number :
26397
Product Name :
pF151 pcDNA3.1(+)SOD1WT
article :
doi10.1371/journal.pone.0009541
id3658
pubmed_id20221404
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1 (+)
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
. The complete cDNA insert was subcloned into the vector pET28 and then a PCR amplified the complete cDNA with EcoRI and XhoI sites on the 5 and 3 end respectively. This EcoRI-Xho1 0.47kb fragment was cloned into the respective EcoRI and XhoI sites of pcDNA3.1(+).
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer
geneSOD1
id6647
genbank_ids
mutation
nameSOD1
shRNA_sequence
size470
species
9606
Homo sapiens
tags
plasmid copy :
human cDNA insert from cDNA clone cSOD1 from Professor Yoram Groner as described in Lieman-Hurwitzet al., 1982 and Sherman et al 1983.
resistance markers :
804
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA