pcDNA3.2/V5 mmu-mir-223 | Addgene 26334 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3.2/V5 mmu-mir-223

catalog :

26334

citations: 2

Reference
Kitayama K, Kawamoto T, Kawakami Y, Hara H, Takemori T, Fujiwara S, et al. Regulatory roles of miRNAs 16, 133a, and 223 on osteoclastic bone destruction caused by breast cancer metastasis. Int J Oncol. 2021;59: pubmed publisher
Chiang H, Schoenfeld L, Ruby J, Auyeung V, Spies N, Baek D, et al. Mammalian microRNAs: experimental evaluation of novel and previously annotated genes. Genes Dev. 2010;24:992-1009 pubmed publisher

product information

Catalog Number :

26334

Product Name :

pcDNA3.2/V5 mmu-mir-223

article :

doi	10.1101/gad.1884710
id	3651
pubmed_id	20413612

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.2/V5-DEST

backbone_mutation

backbone_origin

Invitrogen

backbone_size

7711

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

pre-miRNA hairpins and the surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.

origin :

pi :

alt_names

mir 223

mir-223

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	V5 reverse (5 -ACCGAGGAGAGGGTTAGGGAT-3 )
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Mirn223, miR-223, mmu-mir-223
gene	Mir223
id	723814

genbank_ids

mutation

name

mmu-mir-223

shRNA_sequence

size

357

species

10090

Mus musculus