pcDNA3.2/V5 hsa-mir-499 | Addgene 26320 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3.2/V5 hsa-mir-499

catalog :

26320

citations: 2

Reference
Gulve N, Prusty B, Rudel T. Chlamydia trachomatis impairs host base excision repair by downregulating polymerase β. Cell Microbiol. 2019;21:e12986 pubmed publisher
Chiang H, Schoenfeld L, Ruby J, Auyeung V, Spies N, Baek D, et al. Mammalian microRNAs: experimental evaluation of novel and previously annotated genes. Genes Dev. 2010;24:992-1009 pubmed publisher

product information

Catalog Number :

26320

Product Name :

pcDNA3.2/V5 hsa-mir-499

article :

doi	10.1101/gad.1884710
id	3651
pubmed_id	20413612

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.2/V5-DEST

backbone_mutation

backbone_origin

Invitrogen

backbone_size

7711

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

pre-miRNA hairpins and the surrounding regions were amplified from human genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.

origin :

pi :

alt_names

mir 499

mir-499

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	V5 reverse (5 -ACCGAGGAGAGGGTTAGGGAT-3 )
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MIR499, MIRN499, hsa-mir-499a, mir-499a
gene	MIR499A
id	574501

genbank_ids

mutation

name

hsa-mir-499

shRNA_sequence

size

312

species

9606

Homo sapiens