pcDNA3.2/V5 hsa-mir-124 | Addgene 26306 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3.2/V5 hsa-mir-124

catalog :

26306

citations: 4

Reference
Li X, Yang Z, Han Z, Wen Y, Ma Z, Wang Y. Niclosamide acts as a new inhibitor of vasculogenic mimicry in oral cancer through upregulation of miR-124 and downregulation of STAT3. Oncol Rep. 2018;39:827-833 pubmed publisher
Gu X, Meng S, Liu S, Jia C, Fang Y, Li S, et al. miR-124 represses ROCK1 expression to promote neurite elongation through activation of the PI3K/Akt signal pathway. J Mol Neurosci. 2014;52:156-65 pubmed publisher
Asuthkar S, Stepanova V, Lebedeva T, Holterman A, Estes N, Cines D, et al. Multifunctional roles of urokinase plasminogen activator (uPA) in cancer stemness and chemoresistance of pancreatic cancer. Mol Biol Cell. 2013;24:2620-32 pubmed publisher
Chiang H, Schoenfeld L, Ruby J, Auyeung V, Spies N, Baek D, et al. Mammalian microRNAs: experimental evaluation of novel and previously annotated genes. Genes Dev. 2010;24:992-1009 pubmed publisher

product information

Catalog Number :

26306

Product Name :

pcDNA3.2/V5 hsa-mir-124

article :

doi	10.1101/gad.1884710
id	3651
pubmed_id	20413612

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.2/V5-DEST

backbone_mutation

backbone_origin

Invitrogen

backbone_size

7711

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

pre-miRNA hairpins and the surrounding regions were amplified from human genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.

origin :

pi :

alt_names

mir 124

mir-124

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	V5 reverse (5 -ACCGAGGAGAGGGTTAGGGAT-3 )
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MIR124A, MIR124A1, MIRN124-1, MIRN124A1, mir-124-1
gene	MIR124-1
id	406907

genbank_ids

mutation

name

hsa-mir-124

shRNA_sequence

size

443

species

9606

Homo sapiens