This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTomo-Ras
catalog :
26292
citations: 3
Reference
Liu Y, Xin B, Yamamoto M, Goto M, Ooshio T, Kamikokura Y, et al. Generation of combined hepatocellular-cholangiocarcinoma through transdifferentiation and dedifferentiation in p53-knockout mice. Cancer Sci. 2021;112:3111-3124 pubmed publisher
Xin B, Yamamoto M, Fujii K, Ooshio T, Chen X, Okada Y, et al. Critical role of Myc activation in mouse hepatocarcinogenesis induced by the activation of AKT and RAS pathways. Oncogene. 2017;36:5087-5097 pubmed publisher
Marumoto T, Tashiro A, Friedmann Morvinski D, Scadeng M, Soda Y, Gage F, et al. Development of a novel mouse glioma model using lentiviral vectors. Nat Med. 2009;15:110-6 pubmed publisher
product information
Catalog Number :
26292
Product Name :
pTomo-Ras
article :
doi10.1038/nm.1863
id3646
pubmed_id19122659
bacterial resistance :
Ampicillin
cloning :
backbonepTomo
backbone_mutation
backbone_originVerma lab
backbone_size8753
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
From the paper: To construct pTomo H-RasV12, we amplified Flag-tagged H-RasV12 from pGEM H-RasV12 by PCR and inserted it into the BamHI site of the pTomo vector.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoter
sequencing_primer_3IRES-R
sequencing_primer_5dsRed1-
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
geneHRAS
id3265
genbank_ids
mutationG12V
nameHRAS-V12
shRNA_sequence
size627
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
resistance markers :
548
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA