pBPGAL4.2::VP16Uw | Addgene 26228 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBPGAL4.2::VP16Uw

catalog :

26228

citations: 4

Reference
Qiao X, Zhang X, Zhou Z, Guo L, Wu W, Ma S, et al. An insecticide target in mechanoreceptor neurons. Sci Adv. 2022;8:eabq3132 pubmed publisher
Prange R, Thiedmann M, Bhandari A, Mishra N, Sinha A, Häsler R, et al. A Drosophila model of cigarette smoke induced COPD identifies Nrf2 signaling as an expedient target for intervention. Aging (Albany NY). 2018;10:2122-2135 pubmed publisher
Meissner G, Luo S, Dias B, Texada M, Baker B. Sex-specific regulation of Lgr3 in Drosophila neurons. Proc Natl Acad Sci U S A. 2016;113:E1256-65 pubmed publisher
Pfeiffer B, Ngo T, Hibbard K, Murphy C, Jenett A, Truman J, et al. Refinement of tools for targeted gene expression in Drosophila. Genetics. 2010;186:735-55 pubmed publisher

product information

Catalog Number :

26228

Product Name :

pBPGAL4.2::VP16Uw

article :

doi	10.1534/genetics.110.119917
id	3631
pubmed_id	20697123

bacterial resistance :

Chloramphenicol and Ampicillin

cloning :

backbone

pBPGUw

backbone_mutation

backbone_origin

backbone_size

8770

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth temp :

ccdB Survival 2T1 strain (Invitrogen), or equivalent, must be used to propagate plasmids carrying the ccdB gene.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	KpnI
promoter
sequencing_primer_3	GAL4 R: CGGCATCCTTGTTGACGTTAT
sequencing_primer_5	DSCP F: GAGCTCGCCCGGGGATC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YPL248C, GAL81
gene	GAL4
id	855828

genbank_ids

mutation

name

GAL4::VP16

shRNA_sequence

size

684

species

4932

Saccharomyces cerevisiae