I-SceI pB 5UAS::ZfSyGCaMP2 | Addgene 26125 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

I-SceI pB 5UAS::ZfSyGCaMP2

catalog :

26125

citations: 1

Reference
Dreosti E, Odermatt B, Dorostkar M, Lagnado L. A genetically encoded reporter of synaptic activity in vivo. Nat Methods. 2009;6:883-9 pubmed publisher

product information

Catalog Number :

26125

Product Name :

I-SceI pB 5UAS::ZfSyGCaMP2

article :

doi	10.1038/nmeth.1399
id	3618
pubmed_id	19898484

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript II (KS)

backbone_mutation

backbone_origin

Stratagene

backbone_size

3585

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Zebrafish expression

growth notes :

At both ends of the multiple cloning site of the original pBluescript II KS+, a I-SceI recognition site was cloned replacing the BssHII cutting sequence. In the map, ORF frame 3 (1107-3368): zebrafish SyGCaMP2. Also, 5UAS is located 668-1034.

origin :

pi :

alt_names

SyGCaMP2

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5	EcoRV
promoter
sequencing_primer_3
sequencing_primer_5	M13-F
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	zgc:136469, zgc:92837
gene	sypb
id	436774

genbank_ids

mutation

name

Synaptophysin GCaMP2

shRNA_sequence

size

2262

species

7955

Danio rerio

tags

location	C terminal on insert
tag	GCaMP2

plasmid copy :

Reference the following (for GCaMP2) when using this plasmid, along with the original article: Tallini YN, Ohkura M, Choi B-R, Ji J, Imoto K, Doran R, Lee J, Plan P, Wilson J, Xin H-B, Sanbe A, Gulick J, Mathai J, Robbins J, Salama G, Nakai J, Kotlikoff MI (2006) Imaging cellular signals in the heart in vivo: Cardiac expression of the high signal Ca2+ indicator G-CaMP2. Proc. Natl. Acad. Sci. USA 103, 4753-4758.

resistance markers :

702

tags :

High Copy