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product summary
company name :
Addgene
product type :
cDNA
product name :
CMV::ratSyGCaMP2
catalog :
26124
citations: 9
Reference
Andres Alonso M, Ammar M, Butnaru I, Gomes G, Acuña Sanhueza G, Raman R, et al. SIPA1L2 controls trafficking and local signaling of TrkB-containing amphisomes at presynaptic terminals. Nat Commun. 2019;10:5448 pubmed publisher
Schwarz Y, Oleinikov K, Schindeldecker B, Wyatt A, Weißgerber P, Flockerzi V, et al. TRPC channels regulate Ca2+-signaling and short-term plasticity of fast glutamatergic synapses. PLoS Biol. 2019;17:e3000445 pubmed publisher
Heck J, Parutto P, Ciuraszkiewicz A, Bikbaev A, Freund R, Mitlöhner J, et al. Transient Confinement of CaV2.1 Ca2+-Channel Splice Variants Shapes Synaptic Short-Term Plasticity. Neuron. 2019;: pubmed publisher
Servián Morilla E, Robles Lanuza E, Sánchez Hidalgo A, Camacho Garcia R, Paez Gomez J, Mavillard F, et al. Proteolytic Processing of Neurexins by Presenilins Sustains Synaptic Vesicle Release. J Neurosci. 2018;38:901-917 pubmed publisher
Vaccaro V, Devine M, Higgs N, Kittler J. Miro1-dependent mitochondrial positioning drives the rescaling of presynaptic Ca2+ signals during homeostatic plasticity. EMBO Rep. 2017;18:231-240 pubmed publisher
Al Awabdh S, Gupta Agarwal S, Sheehan D, Muir J, Norkett R, Twelvetrees A, et al. Neuronal activity mediated regulation of glutamate transporter GLT-1 surface diffusion in rat astrocytes in dissociated and slice cultures. Glia. 2016;64:1252-64 pubmed publisher
Hammond J, Lu S, Gelbard H. Platelet Activating Factor Enhances Synaptic Vesicle Exocytosis Via PKC, Elevated Intracellular Calcium, and Modulation of Synapsin 1 Dynamics and Phosphorylation. Front Cell Neurosci. 2015;9:505 pubmed publisher
Herman M, Ackermann F, Trimbuch T, Rosenmund C. Vesicular glutamate transporter expression level affects synaptic vesicle release probability at hippocampal synapses in culture. J Neurosci. 2014;34:11781-91 pubmed publisher
Dreosti E, Odermatt B, Dorostkar M, Lagnado L. A genetically encoded reporter of synaptic activity in vivo. Nat Methods. 2009;6:883-9 pubmed publisher
product information
Catalog Number :
26124
Product Name :
CMV::ratSyGCaMP2
article :
doi10.1038/nmeth.1399
id3618
pubmed_id19898484
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1 (Clontech)
backbone_mutation
backbone_origin
backbone_size3957
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
ORF frame 3 (624-2915): Rat SyGCaMP2. Our sequence with CMV-F shows a mutation S199T in Synaptophysin ORF. This change does not have any effect in localizing the construct to the synaptic vesicle and may be a result of a SNP.
origin :
37
pi :
alt_names
SyGCaMP2
cloning
clone_methodRestriction Enzyme
cloning_site_3Ssp1
cloning_site_5AfeI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesSyp1
geneSyp
id24804
genbank_ids
mutation
nameSynaptophysin GCaMP2
shRNA_sequence
size2292
species
10116
Rattus norvegicus
tags
plasmid copy :
Reference the following (for GCaMP2) when using this plasmid, along with the original article: Tallini YN, Ohkura M, Choi B-R, Ji J, Imoto K, Doran R, Lee J, Plan P, Wilson J, Xin H-B, Sanbe A, Gulick J, Mathai J, Robbins J, Salama G, Nakai J, Kotlikoff MI (2006) Imaging cellular signals in the heart in vivo: Cardiac expression of the high signal Ca2+ indicator G-CaMP2. Proc. Natl. Acad. Sci. USA 103, 4753-4758
resistance markers :
702
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA