pNIC-CH | Addgene 26117 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pNIC-CH

catalog :

26117

citations: 6

Reference
Li Y, Zhao Y, Yan X, Ye C, Weirich S, Zhang B, et al. CRL2ZER1/ZYG11B recognizes small N-terminal residues for degradation. Nat Commun. 2022;13:7636 pubmed publisher
Ashok Y, Vela Rodr xed guez C, Yang C, Alanen H, Liu F, Paschal B, et al. Reconstitution of the DTX3L-PARP9 complex reveals determinants for high-affinity heterodimerization and multimeric assembly. Biochem J. 2022;479:289-304 pubmed publisher
Yan X, Li Y, Wang G, Zhou Z, Song G, Feng Q, et al. Molecular basis for recognition of Gly/N-degrons by CRL2ZYG11B and CRL2ZER1. Mol Cell. 2021;: pubmed publisher
Zhang K, Law Y, Law M, Tan Y, Wirawan M, Luo D. Structural insights into viral RNA capping and plasma membrane targeting by Chikungunya virus nonstructural protein 1. Cell Host Microbe. 2021;29:757-764.e3 pubmed publisher
McNamara D, Dovey C, Hale A, Quarato G, Grace C, Guibao C, et al. Direct Activation of Human MLKL by a Select Repertoire of Inositol Phosphate Metabolites. Cell Chem Biol. 2019;26:863-877.e7 pubmed publisher
Naas A, Solden L, Norbeck A, Brewer H, Hagen L, Heggenes I, et al. "Candidatus Paraporphyromonas polyenzymogenes" encodes multi-modular cellulases linked to the type IX secretion system. Microbiome. 2018;6:44 pubmed publisher

product information

Catalog Number :

26117

Product Name :

pNIC-CH

article :

doi
id	3614
pubmed_id

bacterial resistance :

Kanamycin

cloning :

backbone

pNIC-CH

backbone_mutation

backbone_origin

SGC Oxford

backbone_size

7218

promoter

T7-lacO

sequencing_primer_3

T7-term

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

pET expression vector with C-terminal His6 tag. Includes sites for LIC cloning, and a stuffer fragment that includes the SacB gene, allowing negative selection on 5% sucrose. GenBank accession number: EF199843 Primers for LIC cloning: Add the following 5 extensions to the PCR primers: Upstream: TTAAGAAGGAGATATACTATG (ATG-initiation codon) Downstream: AATGGTGGTGATGATGGTGCGC The purified PCR fragments are treated with T4 DNA polymerase and dGTP, then annealed to the treated vector. See supplemental document for more information.

growth strain :

SGC Empty backbone for bacterial expression

origin :

resistance markers :

791

tags :

Low Copy