pFB-LIC-Bse | Addgene 26108 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pFB-LIC-Bse

catalog :

26108

citations: 3

Reference
Blackburn M, Hubbard C, Kiessling V, Bi Y, Kloss B, TAMM L, et al. Distinct reaction mechanisms for hyaluronan biosynthesis in different kingdoms of life. Glycobiology. 2017;: pubmed publisher
Qian X, Hamid F, El Sahili A, Darwis D, Wong Y, Bhushan S, et al. Functional Evolution in Orthologous Cell-encoded RNA-dependent RNA Polymerases. J Biol Chem. 2016;291:9295-309 pubmed publisher
Savitsky P, Bray J, Cooper C, Marsden B, Mahajan P, Burgess Brown N, et al. High-throughput production of human proteins for crystallization: the SGC experience. J Struct Biol. 2010;172:3-13 pubmed publisher

product information

Catalog Number :

26108

Product Name :

pFB-LIC-Bse

article :

doi	10.1016/j.jsb.2010.06.008
id	3610
pubmed_id	20541610

bacterial resistance :

Ampicillin

cloning :

backbone

pFastBac

backbone_mutation

backbone_origin

Invitrogen

backbone_size

4803

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

Other

baculovirus expression

growth notes :

Primers for LIC cloning: Upstream: add TACTTCCAATCCATG to the 5 end (ATG in-frame with the desired coding sequence). Downstream: add TATCCACCTTTACTG to 5 end of downstream primer; add termination codon, if necessary. Detailed cloning method available in the paper (Savitsky et al., J Struct Biol., 2010). Generate bacmids and baculoviruses using the Bac to Bac method (Invitrogen)

growth strain :

SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

growth temp :

Any E. coli cloning host. To generate bacmids, transform DH10Bac according to the manufacturer's procedure (Invitrogen)

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	polyhedrin
sequencing_primer_3	fBac-2 (5'-GGGAGGTTTTTTAAAGCAAGTAAA-3')
sequencing_primer_5	fBac-1 (5'-TATTCATACCGTCCCACCA-3')
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

genbank_ids

EF199842

mutation

name

shRNA_sequence

size

species