pNH-TrxT | Addgene 26106 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pNH-TrxT

catalog :

26106

citations: 8

Reference
Mistry H, Kumari S, Aswal V, Gupta G. Structural characterization of transcription-coupled repair protein UVSSA and its interaction with TFIIH protein. Int J Biol Macromol. 2023;247:125792 pubmed publisher
Mistry H, Gupta G. Transcription coupled DNA repair protein UVSSA binds to DNA and RNA: Mapping of nucleic acid interaction sites on human UVSSA. Arch Biochem Biophys. 2023;:109515 pubmed publisher
Ashok Y, Vela Rodr xed guez C, Yang C, Alanen H, Liu F, Paschal B, et al. Reconstitution of the DTX3L-PARP9 complex reveals determinants for high-affinity heterodimerization and multimeric assembly. Biochem J. 2022;479:289-304 pubmed publisher
Russo L, Tomasin R, Matos I, Manucci A, Sowa S, Dale K, et al. The SARS-CoV-2 Nsp3 macrodomain reverses PARP9/DTX3L-dependent ADP-ribosylation induced by interferon signalling. J Biol Chem. 2021;:101041 pubmed publisher
Pachauri S, Gupta G, Mukherjee P, Kumar V. Expression of a heptelidic acid-insensitive recombinant GAPDH from Trichoderma virens, and its biochemical and biophysical characterization. Protein Expr Purif. 2020;175:105697 pubmed publisher
Correddu D, Montaño López J, Angermayr S, Middleditch M, Payne L, Leung I. Effect of consecutive rare codons on the recombinant production of human proteins in Escherichia coli. IUBMB Life. 2020;72:266-274 pubmed publisher
Juettner V, Kruse K, Dan A, Vu V, Khan Y, Le J, et al. VE-PTP stabilizes VE-cadherin junctions and the endothelial barrier via a phosphatase-independent mechanism. J Cell Biol. 2019;218:1725-1742 pubmed publisher
Savitsky P, Bray J, Cooper C, Marsden B, Mahajan P, Burgess Brown N, et al. High-throughput production of human proteins for crystallization: the SGC experience. J Struct Biol. 2010;172:3-13 pubmed publisher

product information

Catalog Number :

26106

Product Name :

pNH-TrxT

article :

doi	10.1016/j.jsb.2010.06.008
id	3610
pubmed_id	20541610

bacterial resistance :

Kanamycin

cloning :

backbone

pET-28-a

backbone_mutation

backbone_origin

Novagen

backbone_size

5671

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Primers for LIC cloning: Upstream: add TACTTCCAATCCATG to the 5 end (ATG in-frame with the desired coding sequence). Downstream: add TATCCACCTTTACTG to 5 end of downstream primer; add termination codon, if necessary. Detailed cloning method available in the paper (Savitsky et al., J Struct Biol., 2010)

growth strain :

SGC Empty backbone for bacterial expression

growth temp :

For plasmid propagation and cloning, use any E. coli strain that does not express T7 RNA polymerase. For protein expression, use a host that expresses T7 RNA polymerase, e.g. BL21(DE3).

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Ligation-Independent cloning; cut with BsaI
cloning_site_5	Ligation-Independent cloning; cut with BsaI
promoter
sequencing_primer_3	T7R
sequencing_primer_5	T7F
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

genbank_ids

GU269914

mutation

name

shRNA_sequence

size

species