pNIC-CTHF | Addgene 26105 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pNIC-CTHF

catalog :

26105

citations: 2

Reference
Kotov V, Bartels K, Veith K, Josts I, Subhramanyam U, Günther C, et al. High-throughput stability screening for detergent-solubilized membrane proteins. Sci Rep. 2019;9:10379 pubmed publisher
Savitsky P, Bray J, Cooper C, Marsden B, Mahajan P, Burgess Brown N, et al. High-throughput production of human proteins for crystallization: the SGC experience. J Struct Biol. 2010;172:3-13 pubmed publisher

product information

Catalog Number :

26105

Product Name :

pNIC-CTHF

article :

doi	10.1016/j.jsb.2010.06.008
id	3610
pubmed_id	20541610

bacterial resistance :

Kanamycin

cloning :

backbone

pET-28-a

backbone_mutation

backbone_origin

Novagen

backbone_size

5330

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Primers for LIC cloning: Upstream: add TTAAGAAGGAGATATACTATG to the 5 end (ATG in-frame with the desired coding sequence). Downstream: add GATTGGAAGTAGAGGTTCTCTGC to 5 end of downstream primer (no termination codon!) Detailed cloning method available in the paper (Savitsky et al., J Struct Biol., 2010)

growth strain :

SGC Empty backbone for bacterial expression

growth temp :

For plasmid propagation and cloning use any E. coli strain not expressing T7 RNA polymerase. For expression, use a strain that expresses T7 RNA polymerase, e.g. BL21(DE3).

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Ligation-Independent cloning; cut with BfuAI
cloning_site_5	Ligation-Independent cloning; cut with BfuAI
promoter
sequencing_primer_3	T7R
sequencing_primer_5	T7F
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

genbank_ids

EF199844

mutation

name

shRNA_sequence

size

species