This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNIC28-Bsa4
catalog :
26103
citations: 29
Reference
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Mistry H, Gupta G. Transcription coupled DNA repair protein UVSSA binds to DNA and RNA: Mapping of nucleic acid interaction sites on human UVSSA. Arch Biochem Biophys. 2023;:109515 pubmed publisher
Morreale F, Kleine S, Leodolter J, Junker S, Hoi D, Ovchinnikov S, et al. BacPROTACs mediate targeted protein degradation in bacteria. Cell. 2022;185:2338-2353.e18 pubmed publisher
Kosgei A, Miller M, Bhardwaj M, Xu W, Thorson J, Van Lanen S, et al. The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina. Acta Crystallogr F Struct Biol Commun. 2022;78:1-7 pubmed publisher
Siejka Zielinska P, Cheng J, Jackson F, Liu Y, Soonawalla Z, Reddy S, et al. Cell-free DNA TAPS provides multimodal information for early cancer detection. Sci Adv. 2021;7:eabh0534 pubmed publisher
Ladha F, Thakar K, Pettinato A, Legere N, Ghahremani S, Cohn R, et al. Actinin BioID reveals sarcomere crosstalk with oxidative metabolism through interactions with IGF2BP2. Cell Rep. 2021;36:109512 pubmed publisher
Cheng J, Siejka Zielinska P, Liu Y, Chandran A, Kriaucionis S, Song C. Endonuclease enrichment TAPS for cost-effective genome-wide base-resolution DNA methylation detection. Nucleic Acids Res. 2021;49:e76 pubmed publisher
Liu Y, Hu Z, Cheng J, Siejka Zielinska P, Chen J, Inoue M, et al. Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution. Nat Commun. 2021;12:618 pubmed publisher
Hayes M, O Brien J, Crawford R, Fowler C, Yu L, Doorn J, et al. Fragment-Based Nuclear Magnetic Resonance Screen against a Regulator of G Protein Signaling Identifies a Binding "Hot Spot". Chembiochem. 2021;22:1609-1620 pubmed publisher
Liu W, Li W, Pan X, Sha H, Gong W, Yan M. HBP1 deficiency protects against stress-induced premature senescence of nucleus pulposus. Eur Rev Med Pharmacol Sci. 2020;24:8685-8693 pubmed publisher
Shcherbakova L, Rozhkova A, Osipov D, Zorov I, Mikityuk O, Statsyuk N, et al. Effective Zearalenone Degradation in Model Solutions and Infected Wheat Grain Using a Novel Heterologous Lactonohydrolase Secreted by Recombinant Penicillium canescens. Toxins (Basel). 2020;12: pubmed publisher
Sowa S, Vela Rodríguez C, Galera Prat A, Cázares Olivera M, Prunskaite Hyyryläinen R, Ignatev A, et al. A FRET-based high-throughput screening platform for the discovery of chemical probes targeting the scaffolding functions of human tankyrases. Sci Rep. 2020;10:12357 pubmed publisher
Thomas C, Braun D, Olmos J, Rajski S, Phillips G, Andes D, et al. Pyridine-2,6-Dithiocarboxylic Acid and Its Metal Complexes: New Inhibitors of New Delhi Metallo β-Lactamase-1. Mar Drugs. 2020;18: pubmed publisher
Liu Y, Cheng J, Siejka Zielinska P, Weldon C, Roberts H, Lopopolo M, et al. Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS. Genome Biol. 2020;21:54 pubmed publisher
Chen Z, Picaud S, Filippakopoulos P, D Angiolella V, Bullock A. Structural Basis for Recruitment of DAPK1 to the KLHL20 E3 Ligase. Structure. 2019;: pubmed publisher
McNamara D, Dovey C, Hale A, Quarato G, Grace C, Guibao C, et al. Direct Activation of Human MLKL by a Select Repertoire of Inositol Phosphate Metabolites. Cell Chem Biol. 2019;26:863-877.e7 pubmed publisher
Dalal K, Ban F, Li H, Morin H, Roshan Moniri M, Tam K, et al. Selectively targeting the dimerization interface of human androgen receptor with small-molecules to treat castration-resistant prostate cancer. Cancer Lett. 2018;437:35-43 pubmed publisher
Rouse S, Stylianou F, Wu H, Berry J, Sewell L, Morgan R, et al. The FapF Amyloid Secretion Transporter Possesses an Atypical Asymmetric Coiled Coil. J Mol Biol. 2018;430:3863-3871 pubmed publisher
Zheng J, Grace C, Guibao C, McNamara D, Llambi F, Wang Y, et al. Intrinsic Instability of BOK Enables Membrane Permeabilization in Apoptosis. Cell Rep. 2018;23:2083-2094.e6 pubmed publisher
Costeira Paulo J, Gault J, Popova G, Ladds M, van Leeuwen I, Sarr M, et al. Lipids Shape the Electron Acceptor-Binding Site of the Peripheral Membrane Protein Dihydroorotate Dehydrogenase. Cell Chem Biol. 2018;25:309-317.e4 pubmed publisher
Sharma M, Gupta G, Kumar V. Receptor protein of Lysinibacillus sphaericus mosquito-larvicidal toxin displays amylomaltase activity. Insect Biochem Mol Biol. 2018;93:37-46 pubmed publisher
Gati C, Stetsenko A, Slotboom D, Scheres S, Guskov A. The structural basis of proton driven zinc transport by ZntB. Nat Commun. 2017;8:1313 pubmed publisher
Hayes M, Bodle C, Roman D. Evaluation of the Selectivity and Cysteine Dependence of Inhibitors across the Regulator of G Protein-Signaling Family. Mol Pharmacol. 2018;93:25-35 pubmed publisher
Gahloth D, Heaven G, Jowitt T, Mould A, Bella J, Baldock C, et al. The open architecture of HD-PTP phosphatase provides new insights into the mechanism of regulation of ESCRT function. Sci Rep. 2017;7:9151 pubmed publisher
Gahloth D, Levy C, Walker L, Wunderley L, Mould A, Taylor S, et al. Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP. Structure. 2017;25:1011-1024.e4 pubmed publisher
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Savitsky P, Bray J, Cooper C, Marsden B, Mahajan P, Burgess Brown N, et al. High-throughput production of human proteins for crystallization: the SGC experience. J Struct Biol. 2010;172:3-13 pubmed publisher
product information
Catalog Number :
26103
Product Name :
pNIC28-Bsa4
article :
doi10.1016/j.jsb.2010.06.008
id3610
pubmed_id20541610
bacterial resistance :
Kanamycin
cloning :
backbonepET28a
backbone_mutation
backbone_originNovagen
backbone_size5353
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Primers for LIC cloning: Upstream: add TACTTCCAATCCATG to the 5 end (ATG in-frame with the desired coding sequence). Downstream: add TATCCACCTTTACTG to 5 end of downstream primer; add termination codon, if necessary. Detailed cloning method available in the paper (Savitsky et al., J Struct Biol., 2010)
growth strain :
SGC Empty backbone for bacterial expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Ligation-Independent cloning
cloning_site_5Ligation-Independent cloning
promoter
sequencing_primer_3T7R
sequencing_primer_5T7F
site_3_destroyed1
site_5_destroyed1
entrez_gene
genbank_ids
EF198106
mutation
name
shRNA_sequence
size
species
tags
locationN terminal on backbone
tagHis - TEV
resistance markers :
791
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA