pET28-MHL | Addgene 26096 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET28-MHL

catalog :

26096

citations: 10

Reference
He Q, Lei X, Liu Y, Wang X, Ji N, Yin H, et al. Nucleic Acid Detection through RNA-Guided Protease Activity in Type III-E CRISPR-Cas Systems. Chembiochem. 2023;24:e202300401 pubmed publisher
Guegueniat J, Halabelian L, Zeng H, Dong A, Li Y, Wu H, et al. The human pseudouridine synthase PUS7 recognizes RNA with an extended multi-domain binding surface. Nucleic Acids Res. 2021;49:11810-11822 pubmed publisher
Yan X, Li Y, Wang G, Zhou Z, Song G, Feng Q, et al. Molecular basis for recognition of Gly/N-degrons by CRL2ZYG11B and CRL2ZER1. Mol Cell. 2021;: pubmed publisher
Gurzau A, Horne C, Mok Y, Iminitoff M, Willson T, Young S, et al. SMCHD1's ubiquitin-like domain is required for N-terminal dimerization and chromatin localization. Biochem J. 2021;478:2555-2569 pubmed publisher
Fukuda Y, Pazyra Murphy M, Silagi E, Tasdemir Yilmaz O, Li Y, Rose L, et al. Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors promotes axon survival. J Cell Biol. 2021;220: pubmed publisher
Heinkel F, Shen L, Richard Greenblatt M, Okon M, Bui J, Gee C, et al. Biophysical Characterization of the Tandem FHA Domain Regulatory Module from the Mycobacterium tuberculosis ABC Transporter Rv1747. Structure. 2018;26:972-986.e6 pubmed publisher
Shrestha R, Huang G, Meekins D, Geisbrecht B, Li P. Mechanistic Insights into Dye-Decolorizing Peroxidase Revealed by Solvent Isotope and Viscosity Effects. ACS Catal. 2017;7:6352-6364 pubmed publisher
Xu C, Liu K, Lei M, Yang A, Li Y, Hughes T, et al. DNA Sequence Recognition of Human CXXC Domains and Their Structural Determinants. Structure. 2018;26:85-95.e3 pubmed publisher
Blazer L, Lima Fernandes E, Gibson E, Eram M, Loppnau P, Arrowsmith C, et al. PR Domain-containing Protein 7 (PRDM7) Is a Histone 3 Lysine 4 Trimethyltransferase. J Biol Chem. 2016;291:13509-19 pubmed publisher
Jia K, Cao R, Hua D, Li P. Study of Class I and Class III Polyhydroxyalkanoate (PHA) Synthases with Substrates Containing a Modified Side Chain. Biomacromolecules. 2016;17:1477-85 pubmed publisher

product information

Catalog Number :

26096

Product Name :

pET28-MHL

article :

doi
id	3609
pubmed_id

bacterial resistance :

Kanamycin

cloning :

backbone

pET28-MHL

backbone_mutation

backbone_origin

Structural Genomics Consortium

backbone_size

7325

promoter

T7-lacO

sequencing_primer_3

T7-term

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

The pET28-MHL vector (GenBank accession EF456735) was derived from expression plasmid pET28a-LIC (SGC). It is used for T7 promoter driven expression of recombinant proteins with the addition of an 18 amino acid N-terminal fusion tag containing 6X His followed by a TEV cleavage site. The GSS residues after the Met start site were removed to reduce N-terminal gluconoylation via preventing N-terminal Met excision. Two stop codons are included in the vector at the C-terminal cloning site. Insertion of DNA sequence into the cloning/expression region is preformed using BD-Biosciences Infusion enzyme mediated directional recombination between complementary 15 nucleotide DNA sequences at the ends of the insert (PCR product) and BseRI linearized vector. Insertion of target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose. http://www.sgc.utoronto.ca/SGC-WebPages/toronto-vectors.php

growth strain :

SGC Empty backbone for bacterial expression

origin :

resistance markers :

732

tags :

Low Copy