p15TV-L | Addgene 26093 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

p15TV-L

catalog :

26093

citations: 9

Reference
Diep P, Kell B, Yakunin A, Hilfinger A, Mahadevan R. Quantifying metal-binding specificity of CcNikZ-II from Clostridium carboxidivorans in the presence of competing metal ions. Anal Biochem. 2023;676:115182 pubmed publisher
Saha S, Ojobor C, Li A, Mackinnon E, North O, Bondy Denomy J, et al. F-Type Pyocins Are Diverse Noncontractile Phage Tail-Like Weapons for Killing Pseudomonas aeruginosa. J Bacteriol. 2023;205:e0002923 pubmed publisher
Picott K, Flick R, Edwards E. Heterologous expression of active Dehalobacter spp. respiratory reductive dehalogenases in Escherichia coli. Appl Environ Microbiol. 2021;:AEM0199321 pubmed publisher
Fenton K, Meneely K, Wu T, Martin T, Swint Kruse L, Fenton A, et al. Substitutions at a rheostat position in human aldolase A cause a shift in the conformational population. Protein Sci. 2021;: pubmed publisher
Raj K, Venayak N, Diep P, Golla S, Yakunin A, Mahadevan R. Automation assisted anaerobic phenotyping for metabolic engineering. Microb Cell Fact. 2021;20:184 pubmed publisher
Mejdani M, Pawluk A, Maxwell K, Davidson A. Anti-CRISPR AcrIE2 Binds the Type I-E CRISPR-Cas Complex But Does Not Block DNA Binding. J Mol Biol. 2021;433:166759 pubmed publisher
Diep P, Mahadevan R, Yakunin A. A microplate screen to estimate metal-binding affinities of metalloproteins. Anal Biochem. 2020;609:113836 pubmed publisher
Pagliai F, Pan L, Silva D, Gonzalez C, Lorca G. Zinc is an inhibitor of the LdtR transcriptional activator. PLoS ONE. 2018;13:e0195746 pubmed publisher
Wang P, Khusnutdinova A, Luo F, Xiao J, Nemr K, Flick R, et al. Biosynthesis and Activity of Prenylated FMN Cofactors. Cell Chem Biol. 2018;25:560-570.e6 pubmed publisher

product information

Catalog Number :

26093

Product Name :

p15TV-L

article :

doi
id	3609
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

p15TV-L

backbone_mutation

Derived from pET15b. Used for T7 promoter driven expression of recombinant proteins with the addition of a 21 amino acid N-terminal fusion tag with 6X His followed by a TEV cleavage site. Two stop codons are included at C-terminal cloning site.

backbone_origin

SGC

backbone_size

7746

promoter

T7-lacO

sequencing_primer_3

T7-term

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

The p15TV-L vector (GenBank ID: EF456736.1) is a derivative of pET15b (Novagen) and is for infusion based cloning. It is used for T7 promoter driven expression of recombinant proteins with the addition of a 21 amino acid N-terminal fusion tag with 6X His followed by a TEV cleavage site. Two stop codons are included at C-terminal cloning site. Insertion of DNA sequence into the cloning/expression region is preformed using BD-Biosciences Infusion enzyme mediated directional recombination between complementary 15 nucleotide DNA sequences at the ends of the insert (PCR product) and BseRI linearized vector. Insertion of target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose. http://www.sgc.utoronto.ca/SGC-WebPages/toronto-vectors.php

growth strain :

SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

origin :

resistance markers :

732

tags :

Low Copy