pET15-MHL | Addgene 26092 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET15-MHL

catalog :

26092

citations: 4

Reference
Russell C, Carter J, Borgia J, Bush J, Calder xf3 n F, Gabarr xf3 R, et al. Bromodomain Factor 5 as a Target for Antileishmanial Drug Discovery. ACS Infect Dis. 2023;9:2340-2357 pubmed publisher
Holl xf3 A, Billington N, Takagi Y, Kengyel A, Sellers J, Liu R. Molecular regulatory mechanism of human myosin-7a. J Biol Chem. 2023;299:105243 pubmed publisher
Andrusiak M, Sharifnia P, Lyu X, Wang Z, Dickey A, Wu Z, et al. Inhibition of Axon Regeneration by Liquid-like TIAR-2 Granules. Neuron. 2019;104:290-304.e8 pubmed publisher
Rajiv C, Jackson S, Cocklin S, Eisenmesser E, Davis T. The spliceosomal proteins PPIH and PRPF4 exhibit bi-partite binding. Biochem J. 2017;474:3689-3704 pubmed publisher

product information

Catalog Number :

26092

Product Name :

pET15-MHL

article :

doi
id	3609
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pET15-MHL

backbone_mutation

Derived from pET15. Contains an 18 amino acid N-terminal fusion tag consisting of a 6X His followed by a TEV cleavage site. The GSS residues after the Met start site were removed to reduce N-terminal gluconoylation via preventing N-terminal Met excision. Two stop codons are included in the vector at the C-terminal cloning site.

backbone_origin

SGC

backbone_size

7737

promoter

T7-lacO

sequencing_primer_3

T7-term

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

The pET15-MHL vector (GenBank ID: EF456738.1) is a derivative of pET15b (Novagen) and is for infusion based cloning. It is used for T7 promoter driven expression of recombinant proteins with the addition of an 18 amino acid N-terminal fusion tag containing 6X His followed by a TEV cleavage site. The GSS residues after the Met start site were removed to reduce N-terminal gluconoylation via preventing N-terminal Met excision. Two stop codons are included in the vector at the C-terminal cloning site. Insertion of DNA sequence into the cloning/expression region is preformed using BD-Biosciences Infusion enzyme mediated directional recombination between complementary 15 nucleotide DNA sequences at the ends of the insert (PCR product) and BseRI linearized vector. Insertion of target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose. http://www.sgc.utoronto.ca/SGC-WebPages/toronto-vectors.php

growth strain :

SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

origin :

resistance markers :

732

tags :

Low Copy