pIS1-mutant RhoA 3'UTR | Addgene 26090 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pIS1-mutant RhoA 3'UTR

catalog :

26090

product information

Catalog Number :

26090

Product Name :

pIS1-mutant RhoA 3'UTR

article :

doi	10.1016/j.cell.2009.03.047
id	2977
pubmed_id	19524507

bacterial resistance :

Ampicillin

cloning :

backbone

pIS1

backbone_mutation

backbone_origin

David Bartel, Addgene plasmid # 12179

backbone_size

4085

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

Renilla luciferase reporter controlled by RhoA 3'UTR containing site-directed mutagenized miR-31 binding sites. There is also a G->A mutation in the insert. The Weinberg lab has determined that this mutation does not affection function.

origin :

pi :

alt_names

mutant RhoA reporter

RhoA

cloning

clone_method	Restriction Enzyme
cloning_site_3	AgeI
cloning_site_5	SacI
promoter
sequencing_primer_3	EBV-rev
sequencing_primer_5	Rluc-F (ccaggattcttttccaatgc)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ARH12, ARHA, EDFAOB, RHO12, RHOH12
gene	RHOA
id	387

genbank_ids

mutation

Both miR-31 binding sites in 3 UTR mutagenized. Site 1: from ATCTTGC to AGGCGGC. Site 2: from TCTTGC to CGCCGC.

name

mutant RhoA 3'UTR

shRNA_sequence

size

1061

species

9606

Homo sapiens