This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pIS1-wt RhoA 3'UTR
catalog :
26089
product information
Catalog Number :
26089
Product Name :
pIS1-wt RhoA 3'UTR
article :
doi10.1016/j.cell.2009.03.047
id2977
pubmed_id19524507
bacterial resistance :
Ampicillin
cloning :
backbonepIS1
backbone_mutation
backbone_originDavid Bartel, Addgene plasmid # 12179
backbone_size4085
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
Renilla luciferase reporter controlled by RhoA 3'UTR containing wild-type miR-31 binding sites. The miR-31 motif at nucleotides 145-151 of the human RhoA 3 UTR is ATCTTGC. The miR-31 motif spanning nucleotides 303-309 of the RhoA 3 UTR is TCTTGC.
origin :
37
pi :
alt_names
wt RhoA reporter
RhoA
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5SacI
promoter
sequencing_primer_3EBV-rev
sequencing_primer_5Rluc-F (ccaggattcttttccaatgc)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARH12, ARHA, EDFAOB, RHO12, RHOH12
geneRHOA
id387
genbank_ids
mutation
namewt RhoA 3'UTR
shRNA_sequence
size1061
species
9606
Homo sapiens
tags
resistance markers :
568
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA