This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe-puro-mIR-31
catalog :
26088
product information
Catalog Number :
26088
Product Name :
pBabe-puro-mIR-31
article :
doi10.1016/j.cell.2009.03.047
id2977
pubmed_id19524507
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-puro
backbone_mutation
backbone_originWeinberg lab (Addgene#:1764)
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The human miR-31 gene was PCR-amplified from genomic DNA and cloned into pcDNA3.1 (Invitrogen), then subcloned into the BamHI and SalI sites of the pBABE-puro retroviral vector.
origin :
37
pi :
alt_names
miR31
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3pBabe-3
sequencing_primer_5pBabe-5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIRN31, hsa-mir-31, miR-31
geneMIR31
id407035
genbank_ids
mutation
namemiR-31
shRNA_sequence
size400
species
9606
Homo sapiens
tags
resistance markers :
568
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA