This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSVE Grb2 myc 49L
catalog :
26085
citations: 2
Reference
Soskis M, Ho H, Bloodgood B, Robichaux M, Malik A, Ataman B, et al. A chemical genetic approach reveals distinct EphB signaling mechanisms during brain development. Nat Neurosci. 2012;15:1645-54 pubmed publisher
Egan S, Giddings B, Brooks M, Buday L, Sizeland A, Weinberg R. Association of Sos Ras exchange protein with Grb2 is implicated in tyrosine kinase signal transduction and transformation. Nature. 1993;363:45-51 pubmed
product information
Catalog Number :
26085
Product Name :
pSVE Grb2 myc 49L
article :
doi10.1038/363045a0
id3605
pubmed_id8479536
bacterial resistance :
Ampicillin
cloning :
backbonepSVE
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
mutagenesis performed using oligo GGA AAA GAC GGC TTC ATT TTA AAG AAC TAC ATA GAA ATG, which creates a new DraI site and mutates codon 49 from P to L.
origin :
37
pi :
alt_names
Grb2
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13 reverse?
sequencing_primer_5pBABE-3
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2
geneGRB2
id2885
genbank_ids
mutationP49L
nameGrb2 P49L
shRNA_sequence
size650
species
9606
Homo sapiens
tags
locationC terminal on insert
tagmyc
resistance markers :
568
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA