p-cyclinB1-mcherry (1958) | Addgene 26063 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

p-cyclinB1-mcherry (1958)

catalog :

26063

citations: 3

Reference
Olbrich T, Vega Sendino M, Murga M, de Carcer G, Malumbres M, Ortega S, et al. A Chemical Screen Identifies Compounds Capable of Selecting for Haploidy in Mammalian Cells. Cell Rep. 2019;28:597-604.e4 pubmed publisher
Iwahori S, Kalejta R. Phosphorylation of transcriptional regulators in the retinoblastoma protein pathway by UL97, the viral cyclin-dependent kinase encoded by human cytomegalovirus. Virology. 2017;512:95-103 pubmed publisher
Gavet O, Pines J. Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis. Dev Cell. 2010;18:533-43 pubmed publisher

product information

Catalog Number :

26063

Product Name :

p-cyclinB1-mcherry (1958)

article :

doi	10.1016/j.devcel.2010.02.013
id	3601
pubmed_id	20412769

bacterial resistance :

Kanamycin

cloning :

backbone

pmCherry-N1

backbone_mutation

backbone_origin

See note below

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The backbone was derived from pECFP-N1. ECFP was replaced with mCherry. There is a V5A mutation in cycB1. The mutation does not affect localization of the protein or it's degradation.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	HindIII
promoter
sequencing_primer_3	mCherry-R
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CCNB
gene	CCNB1
id	891

genbank_ids

mutation

name

Cyclin B1

shRNA_sequence

size

species

9606

Homo sapiens