pCMX/cyclinB1-GFP (27) | Addgene 26061 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMX/cyclinB1-GFP (27)

catalog :

26061

citations: 5

Reference
Allais A, FitzHarris G. Absence of a robust mitotic timer mechanism in early preimplantation mouse embryos leads to chromosome instability. Development. 2022;149: pubmed publisher
Misek S, Foda B, Dexheimer T, Akram M, Conrad S, Schmidt J, et al. BRAF Inhibitor Resistance Confers Increased Sensitivity to Mitotic Inhibitors. Front Oncol. 2022;12:766794 pubmed publisher
Jin X, Fang Y, Hu Y, Chen J, Liu W, Chen G, et al. Synergistic activity of the histone deacetylase inhibitor trichostatin A and the proteasome inhibitor PS-341 against taxane-resistant ovarian cancer cell lines. Oncol Lett. 2017;13:4619-4626 pubmed publisher
Jones P, Kaiser M, Ghaderi Najafabadi M, McVey D, Beveridge A, Schofield C, et al. The Coronary Artery Disease-associated Coding Variant in Zinc Finger C3HC-type Containing 1 (ZC3HC1) Affects Cell Cycle Regulation. J Biol Chem. 2016;291:16318-27 pubmed publisher
Hagting A, Jackman M, Simpson K, Pines J. Translocation of cyclin B1 to the nucleus at prophase requires a phosphorylation-dependent nuclear import signal. Curr Biol. 1999;9:680-9 pubmed

product information

Catalog Number :

26061

Product Name :

pCMX/cyclinB1-GFP (27)

article :

doi	10.1016/S0960-9822(99)80308-X
id	3599
pubmed_id	10395539

bacterial resistance :

Ampicillin

cloning :

backbone

pCMX

backbone_mutation

backbone_origin

backbone_size

4500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The stop codon of cyclin B1 in the pCMX vector was mutated to a Mfe1 site to link cyclin B1 to MmGFP. Cyclin B1 was fused to the second amino acid residue of MmGFP by a 5 amino acid linker (AGAQF). The construct is cloned as a HindIII-BamHI fragment into pCMX. The Cyclin insert contains a V5A polymorphism which has no functional consequence. More information on MmGFP can be found in the following reference: Zernicka-Goetz, M., Pines, J., M., Ryan, K., Siemering, K.R., Haseloff, J., and Gurdon, J.B. (1996) An indelible lineage marker for Xenopus using a mutated green fluorescent protein.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	HindIII
promoter
sequencing_primer_3	GFP-R
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CCNB
gene	CCNB1
id	891

genbank_ids

mutation

name

Cyclin B1

shRNA_sequence

size

species

9606

Homo sapiens