pCMX/cyclinB1 (17) | Addgene 26060 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMX/cyclinB1 (17)

catalog :

26060

citations: 2

Reference
Mazzolini L, Broban A, Froment C, Burlet Schiltz O, Besson A, Manenti S, et al. Phosphorylation of CDC25A on SER283 in late S/G2 by CDK/cyclin complexes accelerates mitotic entry. Cell Cycle. 2016;15:2742-52 pubmed publisher
Hagting A, Jackman M, Simpson K, Pines J. Translocation of cyclin B1 to the nucleus at prophase requires a phosphorylation-dependent nuclear import signal. Curr Biol. 1999;9:680-9 pubmed

product information

Catalog Number :

26060

Product Name :

pCMX/cyclinB1 (17)

article :

doi	10.1016/S0960-9822(99)80308-X
id	3599
pubmed_id	10395539

bacterial resistance :

Ampicillin

cloning :

backbone

pCMX

backbone_mutation

backbone_origin

backbone_size

4500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

There is a V5A mutation in cycB1. The mutation does not affect localization of the protein or it's degradation. For a wild type source of CyclinB1, please see plasmid 26062.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	HindIII
promoter
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CCNB
gene	CCNB1
id	891

genbank_ids

mutation

name

Cyclin B1

shRNA_sequence

size

species

9606

Homo sapiens