This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
512 pME-miR
catalog :
26032
citations: 2
Reference
Kara N, Wei C, Commanday A, Patton J. miR-27 regulates chondrogenesis by suppressing focal adhesion kinase during pharyngeal arch development. Dev Biol. 2017;429:321-334 pubmed publisher
Nicoli S, Standley C, Walker P, Hurlstone A, Fogarty K, Lawson N. MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis. Nature. 2010;464:1196-200 pubmed publisher
product information
Catalog Number :
26032
Product Name :
512 pME-miR
article :
doi10.1038/nature08889
id3593
pubmed_id20364122
bacterial resistance :
Kanamycin
cloning :
backbonepME
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Entry vector
growth notes :
EF1alpha gene insert (also known as ef1a, accession number NM_131263) encompassing the TATA box, first non-coding exon 1, intron 1 and partial exon 2, 5 of the ATG. There are minor mismatches in the ef1a region. These changes should not affect plasmid function.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3attL2
cloning_site_5attL1
promoter
sequencing_primer_3M13rev
sequencing_primer_5M13pUC-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEFL1-alpha, chunp6927, eef1a, ef1a, ik:tdsubc_2a3, ik:tdsubc_2b3, tdsubc_2a3, wu:fa91c07, wu:fa94b03, wu:fi13b09, xx:tdsubc_2a3, xx:tdsubc_2b3
geneeef1a1l1
id30516
genbank_ids
mutation
nameef1a
shRNA_sequence
size753
species
7955
Danio rerio
tags
resistance markers :
275
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA