This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
T7-p53DD-pcDNA3
catalog :
25989
citations: 7
Reference
Cicardi M, Hallgren J, Mawrie D, Krishnamurthy K, Markandaiah S, Nelson A, et al. C9orf72 poly(PR) mediated neurodegeneration is associated with nucleolar stress. bioRxiv. 2023;: pubmed publisher
Bonjoch L, Soares de Lima Y, D xed az Gay M, Dotti I, Mu xf1 oz J, Moreira L, et al. Unraveling the impact of a germline heterozygous POLD1 frameshift variant in serrated polyposis syndrome. Front Mol Biosci. 2023;10:1119900 pubmed publisher
Liu X, Sato N, Yabushita T, Li J, Jia Y, Tamura M, et al. IMPDH inhibition activates TLR-VCAM1 pathway and suppresses the development of MLL-fusion leukemia. EMBO Mol Med. 2022;:e15631 pubmed publisher
Langer H, Mossakowski A, Sule R, Gomes A, Baar K. Dominant-negative p53-overexpression in skeletal muscle induces cell death and fiber atrophy in rats. Cell Death Dis. 2022;13:716 pubmed publisher
Fujino T, Goyama S, Sugiura Y, Inoue D, Asada S, Yamasaki S, et al. Mutant ASXL1 induces age-related expansion of phenotypic hematopoietic stem cells through activation of Akt/mTOR pathway. Nat Commun. 2021;12:1826 pubmed publisher
Lawrenson K, Li Q, Kar S, Seo J, Tyrer J, Spindler T, et al. Cis-eQTL analysis and functional validation of candidate susceptibility genes for high-grade serous ovarian cancer. Nat Commun. 2015;6:8234 pubmed publisher
Irwin M, Marin M, Phillips A, Seelan R, Smith D, Liu W, et al. Role for the p53 homologue p73 in E2F-1-induced apoptosis. Nature. 2000;407:645-8 pubmed
product information
Catalog Number :
25989
Product Name :
T7-p53DD-pcDNA3
article :
doi10.1038/35036614
id3056
pubmed_id11034215
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5385
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
There is a L383H mutation in the sequence. The mutation should not affect plasmid function.
growth temp :
DH5alpha
origin :
37
pi :
alt_names
TP53, p53
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR I
cloning_site_5BamH I
promoter
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
AB082923
mutationtruncated cDNA encodes truncated protein (aa 300-393) with dominant negative function, deletion mutant is called DD
nametumor protein p53
shRNA_sequence
size291
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagT7
resistance markers :
238
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA