This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
HA-HIF2 P405A 450-572/ 820-870-pBabe-Puro
catalog :
25955
citations: 1
Reference
Yan Q, Bartz S, Mao M, Li L, Kaelin W. The hypoxia-inducible factor 2alpha N-terminal and C-terminal transactivation domains cooperate to promote renal tumorigenesis in vivo. Mol Cell Biol. 2007;27:2092-102 pubmed
product information
Catalog Number :
25955
Product Name :
HA-HIF2 P405A 450-572/ 820-870-pBabe-Puro
article :
doi10.1128/MCB.01514-06
id2219
pubmed_id17220275
bacterial resistance :
Ampicillin
cloning :
backboneHA-pBabe-puro
backbone_mutation
backbone_origin
backbone_size5140
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth temp :
DH5alpha
origin :
37
pi :
alt_names
HIF2a
EPAS1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR I
cloning_site_5BamH I
promoter
sequencing_primer_3pBabe-3'
sequencing_primer_5pBabe-5'
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73
geneEPAS1
id2034
genbank_ids
HSU81984
mutationcDNA changed amino acids: Proline 405 to Alanine internal deletion of Amino acids 450 - 527 (NTAD = N-terminal transactivation domain) deletion of Amino acids 820 - 870 (CTAD = C-terminal transactivation domain) This mutant is called dNC in Yan et al. 2007, Mol Cell Biol, 27, 209-2-102 .
nameHypoxia inducible factor 2 alpha
shRNA_sequence
size2117
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHA-tag
resistance markers :
238
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA