pCherry-FRB | Addgene 25920 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCherry-FRB

catalog :

25920

citations: 4

Reference
Dagliyan O, Dokholyan N, Hahn K. Engineering proteins for allosteric control by light or ligands. Nat Protoc. 2019;14:1863-1883 pubmed publisher
Ray A, Klomp J, Collins K, Karginov A. Dissecting Kinase Effector Signaling Using the RapRTAP Methodology. Methods Mol Biol. 2017;1636:21-33 pubmed publisher
Verbeke S, Richard E, Monceau E, Schmidt X, Rousseau B, Velasco V, et al. Humanization of the mouse mammary gland by replacement of the luminal layer with genetically engineered preneoplastic human cells. Breast Cancer Res. 2014;16:504 pubmed publisher
Karginov A, Ding F, Kota P, Dokholyan N, Hahn K. Engineered allosteric activation of kinases in living cells. Nat Biotechnol. 2010;28:743-7 pubmed publisher

product information

Catalog Number :

25920

Product Name :

pCherry-FRB

article :

doi	10.1038/nbt.1639
id	3564
pubmed_id	20581846

bacterial resistance :

Kanamycin

cloning :

backbone

pCherry

backbone_mutation

backbone_origin

backbone_size

3900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

FRB is a small domain derived from full length mTOR. In brief, rapamycin binds to a protein called FKBP12. Rapamycin bound to FKBP12 inhibits mTOR signaling

origin :

pi :

alt_names

FKBP12-Rapamycin Binding domain of mTOR

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	BglII
promoter
sequencing_primer_3
sequencing_primer_5	CMV-Fw
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
gene	MTOR
id	2475

genbank_ids

mutation

name

FRB

shRNA_sequence

size

360

species

9606

Homo sapiens