This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-FRB
catalog :
25919
citations: 7
Reference
Willette B, Zhang J, Zhang J, Tsvetanova N. Endosome positioning coordinates spatially selective GPCR signaling. Nat Chem Biol. 2023;: pubmed publisher
Kim R, Kim J. Engineered Extracellular Vesicles with Compound-Induced Cargo Delivery to Solid Tumors. Int J Mol Sci. 2023;24: pubmed publisher
Chen Y, Huang T, Lin Y, Lin S, Li W, Hsiao H, et al. VPS34 K29/K48 branched ubiquitination governed by UBE3C and TRABID regulates autophagy, proteostasis and liver metabolism. Nat Commun. 2021;12:1322 pubmed publisher
Kim S, Shin J, Oh H, Ahn S, Kim N, Heo W. An inducible system for in vitro and in vivo Fas activation using FKBP-FRB-rapamycin complex. Biochem Biophys Res Commun. 2019;: pubmed publisher
Hayashi Y, Nemoto Sasaki Y, Matsumoto N, Hama K, Tanikawa T, Oka S, et al. Complex formation of sphingomyelin synthase 1 with glucosylceramide synthase increases sphingomyelin and decreases glucosylceramide levels. J Biol Chem. 2018;293:17505-17522 pubmed publisher
Nakazawa N, Sathe A, Shivashankar G, Sheetz M. Matrix mechanics controls FHL2 movement to the nucleus to activate p21 expression. Proc Natl Acad Sci U S A. 2016;113:E6813-E6822 pubmed
Karginov A, Ding F, Kota P, Dokholyan N, Hahn K. Engineered allosteric activation of kinases in living cells. Nat Biotechnol. 2010;28:743-7 pubmed publisher
product information
Catalog Number :
25919
Product Name :
pEGFP-FRB
article :
doi10.1038/nbt.1639
id3564
pubmed_id20581846
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
FRB is a small domain derived from full length mTOR. In brief, rapamycin binds to a protein called FKBP12. Rapamycin bound to FKBP12 inhibits mTOR signaling
origin :
37
pi :
alt_names
FKBP12-Rapamycin Binding domain of mTOR
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5CMV-Fw
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
geneMTOR
id2475
genbank_ids
mutation
nameFRB
shRNA_sequence
size360
species
9606
Homo sapiens
tags
resistance markers :
185
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA