This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
TU#867 (Punc-119sid-1)
catalog :
25811
citations: 2
Reference
Wabnig S, Liewald J, Yu S, Gottschalk A. High-Throughput All-Optical Analysis of Synaptic Transmission and Synaptic Vesicle Recycling in Caenorhabditis elegans. PLoS ONE. 2015;10:e0135584 pubmed publisher
Calixto A, Chelur D, Topalidou I, Chen X, Chalfie M. Enhanced neuronal RNAi in C. elegans using SID-1. Nat Methods. 2010;7:554-9 pubmed publisher
product information
Catalog Number :
25811
Product Name :
TU#867 (Punc-119sid-1)
article :
doi10.1038/nmeth.1463
id3511
pubmed_id20512143
bacterial resistance :
Ampicillin
cloning :
backbonepPD95.75
backbone_mutation
backbone_originA. Fire
backbone_size4000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
growth notes :
Specific details about this vector are given at the Materials and Methods of the article: Calixto et al, 2010, Nat Meth Enhanced Neuronal RNAi using sid-1 Note that this plasmid contains genomic sid-1, NOT the cDNA.
origin :
37
pi :
alt_names
sid-1
cloning
clone_methodRestriction Enzyme
cloning_site_3PvuI
cloning_site_5BamHI
promoter
sequencing_primer_3ATATAAGCGGCCGCAGAAAGGTGTCATGGTCTAGT
sequencing_primer_5TATAGGATCCTCATTTTTCCAGGTTCACAATG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_C04F5.1
genesid-1
id178900
genbank_ids
mutation
namesystemic RNA interference-deficient 1
shRNA_sequence
size7860
species
6239
Caenorhabditis elegans
tags
resistance markers :
72
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA