This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLL3.7_hsa-miR-150
catalog :
25792
citations: 2
Reference
Chirichella M, Bianchi N, Džafo E, Foli E, Gualdrini F, Kenyon A, et al. RFX transcription factors control a miR-150/PDAP1 axis that restrains the proliferation of human T cells. PLoS Biol. 2022;20:e3001538 pubmed publisher
Navarro F, Gutman D, Meire E, Cáceres M, Rigoutsos I, Bentwich Z, et al. miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53. Blood. 2009;114:2181-92 pubmed publisher
product information
Catalog Number :
25792
Product Name :
pLL3.7_hsa-miR-150
article :
doi10.1182/blood-2009-02-205062
id3506
pubmed_id19584398
bacterial resistance :
Ampicillin
cloning :
backbonepLL3.7
backbone_mutation
backbone_origin
backbone_size7650
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
RNAi
origin :
37
pi :
alt_names
miR-150
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XbaI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIRN150, miRNA150, mir-150
geneMIR150
id406942
aliasesCmyb, c-myb, c-myb_CDS, efg
geneMYB
id4602
genbank_ids
mutation
namehsa-miR-150
shRNA_sequence
size490
species
9606
Homo sapiens
tags
plasmid copy :
The pLL3.7 cassette containing the U6 promoter and a control short hairpin was replaced by a cassette containing the U6 promoter and an approx 500 bp genomic fragment for expression of the mature miRNA from pSIREN-RetroQ-miRNA expression vectors, kindly provided by Dr. B. Ramratnam, Brown University
resistance markers :
291
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA