This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLL3.7_hsa-miR-34a
catalog :
25791
citations: 5
Reference
Wu X, Cheng Y, Matthen M, Yoon A, Schwartz G, Bala S, et al. Down-regulation of the tumor suppressor miR-34a contributes to head and neck cancer by up-regulating the MET oncogene and modulating tumor immune evasion. J Exp Clin Cancer Res. 2021;40:70 pubmed publisher
Anastasiadou E, Stroopinsky D, Alimperti S, Jiao A, Pyzer A, Cippitelli C, et al. Epstein-Barr virus-encoded EBNA2 alters immune checkpoint PD-L1 expression by downregulating miR-34a in B-cell lymphomas. Leukemia. 2019;33:132-147 pubmed publisher
Iwagami Y, Zou J, Zhang H, Cao K, Ji C, Kim M, et al. Alcohol-mediated miR-34a modulates hepatocyte growth and apoptosis. J Cell Mol Med. 2018;: pubmed publisher
Pyzer A, Stroopinsky D, Rajabi H, Washington A, Tagde A, Coll M, et al. MUC1-mediated induction of myeloid-derived suppressor cells in patients with acute myeloid leukemia. Blood. 2017;129:1791-1801 pubmed publisher
Navarro F, Gutman D, Meire E, Cáceres M, Rigoutsos I, Bentwich Z, et al. miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53. Blood. 2009;114:2181-92 pubmed publisher
product information
Catalog Number :
25791
Product Name :
pLL3.7_hsa-miR-34a
article :
doi10.1182/blood-2009-02-205062
id3506
pubmed_id19584398
bacterial resistance :
Ampicillin
cloning :
backbonepLL3.7
backbone_mutation
backbone_origin
backbone_size7650
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
RNAi
origin :
37
pi :
alt_names
miR-34a
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XbaI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutation
namehsa-miR-34a
shRNA_sequence
size260
species
9606
Homo sapiens
tags
plasmid copy :
The pLL3.7 cassette containing the U6 promoter and a control short hairpin was replaced by a cassette containing the U6 promoter and an approx 300 bp genomic fragment for expression of the mature miRNA from pSIREN-RetroQ-miRNA expression vectors, kindly provided by Dr. B. Ramratnam, Brown University
resistance markers :
291
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA