This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFAST NPC1(NTD)-3Q
catalog :
25772
citations: 1
Reference
Kwon H, Abi Mosleh L, Wang M, Deisenhofer J, Goldstein J, Brown M, et al. Structure of N-terminal domain of NPC1 reveals distinct subdomains for binding and transfer of cholesterol. Cell. 2009;137:1213-24 pubmed publisher
product information
Catalog Number :
25772
Product Name :
pFAST NPC1(NTD)-3Q
article :
doi10.1016/j.cell.2009.03.049
id3493
pubmed_id19563754
bacterial resistance :
Ampicillin
cloning :
backbonepFASTBAC
backbone_mutation
backbone_origininvitrogen
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
There is a TEV cleavage site downstream of His tag.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3ECOR1
cloning_site_5SFO1
promoter
sequencing_primer_3SV40pA-R
sequencing_primer_5Polyhedrin forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNPC, POGZ, SLC65A1
geneNPC1
id4864
genbank_ids
mutationaa23-252 only; N70Q N122Q N185Q
namenpc1
shRNA_sequence
size687
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
resistance markers :
110
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA