This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEN_TmiR_G12-E
catalog :
25761
citations: 1
Reference
Shin K, Wall E, Zavzavadjian J, Santat L, Liu J, Hwang J, et al. A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Proc Natl Acad Sci U S A. 2006;103:13759-64 pubmed
product information
Catalog Number :
25761
Product Name :
pEN_TmiR_G12-E
article :
doi10.1073/pnas.0606179103
id3489
pubmed_id16945906
bacterial resistance :
Gentamicin
cloning :
backbonepENTR1A-Gent
backbone_mutation
backbone_originATCC 10326362
backbone_size4259
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Entry vector
growth notes :
Entry vector with TRE-driven G alpha 12 miR-shRNA G alpha 12 miR-shRNA: GCGACACCATCTTCGACAACAT Addgene sequence shows that this plasmid is missing the EcoRI and SpeI sites.
growth strain :
Entry vector with TRE promoter driving mouse G alpha 12 miR30-based shRNA.
growth temp :
Top10
origin :
37
pi :
alt_names
G alpha 12
cloning
clone_methodRestriction Enzyme
cloning_site_3attL2
cloning_site_5attL1
promoter
sequencing_primer_3n/a
sequencing_primer_5pCEP-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGalpha12
geneGna12
id14673
genbank_ids
mutation
nameG alpha 12 miR-shRNA
shRNA_sequence
size22
species
10090
Mus musculus
tags
plasmid copy :
TRE promoter- David Anderson, Caltech; miR30- Greg Hannon, CSHL
resistance markers :
763
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA