This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSLIK-Venus-TmiR-G12
catalog :
25740
citations: 1
Reference
Shin K, Wall E, Zavzavadjian J, Santat L, Liu J, Hwang J, et al. A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Proc Natl Acad Sci U S A. 2006;103:13759-64 pubmed
product information
Catalog Number :
25740
Product Name :
pSLIK-Venus-TmiR-G12
article :
doi10.1073/pnas.0606179103
id3489
pubmed_id16945906
bacterial resistance :
Ampicillin
cloning :
backbonepSLIK
backbone_mutation
backbone_originIain Fraser
backbone_size12370
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
pSLIK-Venus with G alpha 12 miR-shRNA G alpha 12 miR-shRNA: GCGACACCATCTTCGACAACAT
growth strain :
Lentiviral vector with Tet-based inducible expression of mouse G alpha 12 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.
growth temp :
Top10
origin :
37
pi :
alt_names
G alpha 12
cloning
clone_methodRestriction Enzyme
cloning_site_3BfuA1
cloning_site_5BfuA1
promoter
sequencing_primer_3EXFP-R, hUBCpro-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGalpha12
geneGna12
id14673
genbank_ids
mutation
nameG alpha 12 miR-shRNA
shRNA_sequence
size22
species
10090
Mus musculus
tags
locationC terminal on backbone
tagVenus
plasmid copy :
rtTA- Atze Das, University of Amsterdam; TRE promoter- David Anderson, Caltech; miR30- Greg Hannon, CSHL Venus- Tobias Meyer, Stanford
resistance markers :
763
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA