pTZ U6+27 | Addgene 25713 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pTZ U6+27

catalog :

25713

citations: 1

Reference
Good P, Krikos A, Li S, Bertrand E, Lee N, Giver L, et al. Expression of small, therapeutic RNAs in human cell nuclei. Gene Ther. 1997;4:45-54 pubmed

product information

Catalog Number :

25713

Product Name :

pTZ U6+27

article :

doi	10.1038/sj.gt.3300354
id	3476
pubmed_id	9068795

bacterial resistance :

Ampicillin

cloning :

backbone

pTZ18U

backbone_mutation

backbone_origin

Bio-Rad

backbone_size

3000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The U6+27 cassette was constructed by PCR amplification from 265 nucleotides upstream of the human U6 gene transcription initiation site through the first 27 nucleotides of the transcribed gene.

growth strain :

for nuclear expression of small RNAs in mammalian cells

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	M13_puc_F
sequencing_primer_5	hU6-F (5'-GAGGGCCTATTTCCCATGATT-3')
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	RNU6, RNU6A, U6, U6-1
gene	RNU6-1
id	26827

genbank_ids

mutation

name

U6 snRNP

shRNA_sequence

size

species

9606

Homo sapiens