pPGS-hSLUG.fl.flag | Addgene 25696 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pPGS-hSLUG.fl.flag

catalog :

25696

citations: 6

Reference
Vogiatzoglou A, Spanou S, Sachini N, Drakos E, Nikolaou C, Makatounakis T, et al. Promyelocytic leukemia protein regulates angiogenesis and epithelial-mesenchymal transition to limit metastasis in MDA-MB-231 breast cancer cells. Mol Oncol. 2023;17:2090-2108 pubmed publisher
Hensch N, Bondra K, Wang L, Sreenivas P, Zhao X, Modi P, et al. Sensitization to Ionizing Radiation by MEK Inhibition Is Dependent on SNAI2 in Fusion-Negative Rhabdomyosarcoma. Mol Cancer Ther. 2023;22:123-134 pubmed publisher
Vrenken K, Vervoort B, van Ingen Schenau D, Derks Y, van Emst L, Grytsenko P, et al. The transcriptional repressor SNAI2 impairs neuroblastoma differentiation and inhibits response to retinoic acid therapy. Biochim Biophys Acta Mol Basis Dis. 2020;1866:165644 pubmed publisher
Tisza M, Zhao W, Fuentes J, Prijic S, Chen X, Levental I, et al. Motility and stem cell properties induced by the epithelial-mesenchymal transition require destabilization of lipid rafts. Oncotarget. 2016;7:51553-51568 pubmed publisher
Dbouk H, Weil L, Perera G, Dellinger M, Pearson G, Brekken R, et al. Actions of the protein kinase WNK1 on endothelial cells are differentially mediated by its substrate kinases OSR1 and SPAK. Proc Natl Acad Sci U S A. 2014;111:15999-6004 pubmed publisher
Fenouille N, Tichet M, Dufies M, Pottier A, Mogha A, Soo J, et al. The epithelial-mesenchymal transition (EMT) regulatory factor SLUG (SNAI2) is a downstream target of SPARC and AKT in promoting melanoma cell invasion. PLoS ONE. 2012;7:e40378 pubmed publisher

product information

Catalog Number :

25696

Product Name :

pPGS-hSLUG.fl.flag

article :

doi
id	3472
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pPGS-CMV-CITE-Neo(-)

backbone_mutation

backbone_origin

Gary Nabel

backbone_size

7131

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Retroviral

growth notes :

The pPGS-CMV-CITE-neo vector allows the expression of chimeric transcripts encoding a gene of interest fused to the neomycin resistance gene. Expression of the neomycin resistance protein results from an internal ribosome entry site upstream of the neomycin open reading frame.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	NotI
promoter
sequencing_primer_3	T7
sequencing_primer_5	T3
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	SLUG, SLUGH, SLUGH1, SNAIL2, WS2D
gene	SNAI2
id	6591

genbank_ids

mutation

name

SLUG

shRNA_sequence

size

850

species

9606

Homo sapiens