This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MBP-His6-TEV-MMS2
catalog :
25465
citations: 1
Reference
Eddins M, Carlile C, Gomez K, Pickart C, Wolberger C. Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation. Nat Struct Mol Biol. 2006;13:915-20 pubmed
product information
Catalog Number :
25465
Product Name :
MBP-His6-TEV-MMS2
article :
doi10.1038/nsmb1148
id3459
pubmed_id16980971
bacterial resistance :
Ampicillin
cloning :
backbonepMalC2XHV
backbone_mutation
backbone_originmodified from NEB vector
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth temp :
BL21(DE3) RIL (chloramphenicol) 37C growth to O.D.=0.6 induce with 1mM IPTG @16C overnight
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5MalE
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYGL087C
geneMMS2
id852793
aliasesYDR092W
geneUBC13
id851666
genbank_ids
NP_011428
mutation
nameMMS2
shRNA_sequence
size414
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tagMBP
locationN terminal on insert
tagHis6
resistance markers :
583
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA