This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc
catalog :
25458
citations: 1
Reference
Chufan E, De M, Eipper B, Mains R, Amzel L. Amidation of bioactive peptides: the structure of the lyase domain of the amidating enzyme. Structure. 2009;17:965-73 pubmed publisher
product information
Catalog Number :
25458
Product Name :
pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc
article :
doi10.1016/j.str.2009.05.008
id3456
pubmed_id19604476
bacterial resistance :
Ampicillin
cloning :
backbonepCI-neo
backbone_mutation
backbone_originPromega
backbone_size5472
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
peptidyl-alpha-hydroxyglycine alpha-amidating lyase
cloning
clone_methodRestriction Enzyme
cloning_site_3XmaI
cloning_site_5NheI
promoter
sequencing_primer_3T3
sequencing_primer_5T7, CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPHM
genePam
id25508
genbank_ids
mutationContains aa 498-820 (PALcc= PAL catalytic core) Serine 767 mutated to Alanine (delta Glyc)
namePAM
shRNA_sequence
size966
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagPAM signal
locationN terminal on insert
tagHis6
resistance markers :
750
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA