258/-2.5CAT | Addgene 25431 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

258/-2.5CAT

catalog :

25431

citations: 1

Reference
Goldhamer D, Brunk B, Faerman A, King A, Shani M, Emerson C. Embryonic activation of the myoD gene is regulated by a highly conserved distal control element. Development. 1995;121:637-49 pubmed

product information

Catalog Number :

25431

Product Name :

258/-2.5CAT

article :

doi
id	3445
pubmed_id	7720572

bacterial resistance :

Ampicillin

cloning :

backbone

pCATdeltaEH

backbone_mutation

backbone_origin

Mellon Lab

backbone_size

4100

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Other

Reporter construct

growth notes :

The 258/-2.5 core enhancer/promoter fragment can be removed by digestion with SalI or NotI and XhoI. The resulting fragment is approximately 3kb. All tk sequences were removed from the CAT vector in the construction of 258/-2.5CAT. Between the enhancer and promoter is a portion of the pBluescript polylinker tha t includes BamHI, SpeI and SmaI sites, none of which are unique. 258 refers to the core enhancer, which is present in this construct, upstream of the 2.5 kb MyoD promoter

origin :

pi :

alt_names

MyoD

Myo D

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	XbaI
promoter
sequencing_primer_3	CAT-R
sequencing_primer_5	N/A
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	CMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1
gene	MYOD1
id	4654

genbank_ids

mutation

name

Myo D Promoter/Enhancer

shRNA_sequence

size

species

9606

Homo sapiens