F3/-2.5lacZ | Addgene 25430 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

F3/-2.5lacZ

catalog :

25430

citations: 1

Reference
Goldhamer D, Brunk B, Faerman A, King A, Shani M, Emerson C. Embryonic activation of the myoD gene is regulated by a highly conserved distal control element. Development. 1995;121:637-49 pubmed

product information

Catalog Number :

25430

Product Name :

F3/-2.5lacZ

article :

doi
id	3445
pubmed_id	7720572

bacterial resistance :

Ampicillin

cloning :

backbone

pPD46.21

backbone_mutation

backbone_origin

Fire et al., 1990

backbone_size

6110

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Other

Reporter construct

growth notes :

A SalI-XhoI fragment containing the 2.5 kb promoter fragment was cloned into the SalI site of pPD46.21, destroying the XhoI site. The F3 fragment is cloned in reverse orientation. 258 refers to the core enhancer, which is present in this construct, upstream of the 2.5 kb MyoD promoter

origin :

pi :

alt_names

MyoD

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	SalI
promoter
sequencing_primer_3	LacZ-R
sequencing_primer_5	n/A
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	CMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1
gene	MYOD1
id	4654

genbank_ids

mutation

name

Myo D Promoter

shRNA_sequence

size

species

9606

Homo sapiens