This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
258/-2.5lacZ
catalog :
25429
citations: 1
Reference
Goldhamer D, Brunk B, Faerman A, King A, Shani M, Emerson C. Embryonic activation of the myoD gene is regulated by a highly conserved distal control element. Development. 1995;121:637-49 pubmed
product information
Catalog Number :
25429
Product Name :
258/-2.5lacZ
article :
doi
id3445
pubmed_id7720572
bacterial resistance :
Ampicillin
cloning :
backbonepPD46.21
backbone_mutation
backbone_originFire et al., 1990
backbone_size6110
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
Reporter construct
growth notes :
A SalI-XhoI fragment containing the 2.5 kb promoter fragment was cloned into the SalI site of pPD46.21, destroying the XhoI site. 258 refers to the core enhancer, which is present in this construct, upstream of the 2.5 kb MyoD promoter
origin :
37
pi :
alt_names
MyoD
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5SalI
promoter
sequencing_primer_3LacZ-R
sequencing_primer_5n/A
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1
geneMYOD1
id4654
genbank_ids
mutation
nameMyo D Promoter
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
taglacZ
resistance markers :
747
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA