This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
-2.5lacZ
catalog :
25428
citations: 1
Reference
Goldhamer D, Brunk B, Faerman A, King A, Shani M, Emerson C. Embryonic activation of the myoD gene is regulated by a highly conserved distal control element. Development. 1995;121:637-49 pubmed
product information
Catalog Number :
25428
Product Name :
-2.5lacZ
article :
doi
id3445
pubmed_id7720572
bacterial resistance :
Ampicillin
cloning :
backbonepPD46.21
backbone_mutation
backbone_originFire et al., 1990
backbone_size6110
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
Reporter construct
growth notes :
A SalI-XhoI fragment containing the 2.5 kb promoter fragment was cloned into the SalI site of pPD46.21, destroying the XhoI site.
origin :
37
pi :
alt_names
MyoD
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5SalI
promoter
sequencing_primer_3LacZ-R
sequencing_primer_5n/A
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1
geneMYOD1
id4654
genbank_ids
mutation
nameMyo D Promoter
shRNA_sequence
size2700
species
9606
Homo sapiens
tags
locationC terminal on insert
taglacZ
resistance markers :
747
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA